Alpha-smooth muscle actin expression enhances cell traction force
Alpha-smooth muscle actin expression enhances cell traction force
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DOI：
10.1002/cm.20178
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发表时间：
2007-04-01
影响因子：
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通讯作者：
Wang, James H. -C.
中科院分区：
文献类型：
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作者：
Chen, Jianxin;Li, Hongxia;Wang, James H. -C.
使用已建立的角膜基质细胞分化模型，我们通过用TGF-β 1、bFGF、TGF-β I型受体抑制剂（SB-431542）和针对α-平滑肌肌动蛋白（α-SMA）的siRNA处理成纤维细胞来操纵α-SMA蛋白表达水平。通过细胞牵引力显微镜测定相应的细胞牵引力（CTF）。通过所有这些治疗，我们发现α-SMA不是CTF诱导所需的，但其表达上调CTF。这种上调涉及应力纤维的修饰，但似乎与非肌肉肌球蛋白II表达或β-肌动蛋白表达无关。此外，α-SMA蛋白表达水平与CTF幅度之间存在线性关系。最后，发现CTFs在肌成纤维细胞群体中变化，表明单个细胞的α-SMA蛋白表达水平也不同。
Using an established corneal stromal cell differentiation model, we manipulated alpha-smooth muscle actin (alpha-SMA) protein expression levels in fibroblasts by treating them with TGF-beta 1, bFGF, TGF-beta type I receptor inhibitor (SB-431542), and siRNA against alpha-SMA. The corresponding cell traction forces (CTFs) were determined by cell traction force microscopy. With all these treatments, we found that alpha-SMA is not required for CTF induction, but its expression upregulates CTF. This upregulation involves the modification of stress fibers but does not appear to relate to non-muscle myosin II expression or beta-actin expression. Moreover, there exists a linear relationship between alpha-SMA protein expression level and CTF magnitude. Finally, CTFs were found to vary among a population of myofibroblasts, suggesting that alpha-SMA protein expression levels of individual cells also vary.