Effects of time and duration of rearing with bottom sand on the occurrence and expansion of staining-type hypermelanosis in the Japanese flounder Paralichthys olivaceus.
Effects of time and duration of rearing with bottom sand on the occurrence and expansion of staining-type hypermelanosis in the Japanese flounder Paralichthys olivaceus.
复制标题
底砂饲养时间和持续时间对牙鲆染色型黑变病发生和扩大的影响
DOI：
10.1007/s12562-014-0755-5
复制
发表时间：
2014
影响因子：
1.9
通讯作者：
Masatomo Tagawa
中科院分区：
文献类型：
--
作者：
Toshinori Isojima;Naoshi Makino;Yoshifumi Miyama;Masatomo Tagawa
我们之前曾报道，在特定的时间和区域，染色型过度黑素增多症的进展会在特定的时间和区域自发停止。为了检验时间是否是自发停止染色的限制因素，我们通过操纵鱼缸中的底物条件来实验控制染色的开始和持续时间。在孵化后151天(DPH；11周)，观察到在没有沙质底物的水箱中饲养的鱼自发停止染色。然而，在11周后清除沙子的储罐中，染色恢复(或开始)，这表明底部沙子的强烈但暂时的影响，以及151℃DPH时染色进展没有时间限制。由于染色扩大率的增加，延长黑色素沉着扩张的抑制期(9周或更长时间)仅有助于最终染色面积减少20%。底部的沙质底物降低了个体染色区域的可见度，但这只是在染色扩展完成之前观察到的。这些发现与未来染色可能存在的面积限制以及染色的基本性质有关。
We previously reported that the progression of staining-type hypermelanosis spontaneously ceased at a specific time and area in Japanese flounderParalichthys olivaceus. To examine whether time is a limiting factor in the spontaneous cessation of staining, we experimentally controlled the initiation and duration of staining by manipulating the bottom substrate condition in the fish tanks. At 151 days post hatching (DPH; 11 weeks), spontaneous cessation of staining was observed in fish reared in tanks without a sandy substrate. However, staining resumed (or was initiated) in tanks where sand was removed from 11 weeks, indicating a strong but temporary effect of bottom sand and the absence of time limitation in the staining progression by 151 DPH. Extended duration of the inhibitory period of hypermelanosis expansion (9 weeks or more) aided in only a 20 % reduction of the final staining area because of the increased rate of staining expansion. The bottom sandy substrate decreased the visibility of the staining area in individuals, but this was observed only before the completion of the staining expansion. These findings are discussed in relation to possible presence of area limitation of future staining, as well as the fundamental nature of staining.