Isolation and characterization of the proteoglycans synthesized by adult human pulp fibroblasts in vitro.
Isolation and characterization of the proteoglycans synthesized by adult human pulp fibroblasts in vitro.
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DOI：
10.1111/j.1365-2591.1995.tb00292.x
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发表时间：
1995-05
影响因子：
5
通讯作者：
P. Bartold;A. Moule;H. Li;P. Rigby
中科院分区：
文献类型：
--
作者：
P. Bartold;A. Moule;H. Li;P. Rigby
从健康成人牙髓成纤维细胞合成的蛋白多糖已被分离，其特征在于其糖胺聚糖含量，分子大小和电荷的基础上。通过[35S]硫酸盐标记和对木瓜蛋白酶消化的敏感性鉴定蛋白聚糖。与蛋白聚糖相关的硫酸化糖胺聚糖在特定的酶促和化学降解后被鉴定为硫酸软骨素、硫酸皮肤素和硫酸乙酰肝素。硫酸皮肤素、硫酸软骨素和硫酸乙酰肝素是与细胞层相关的主要糖胺聚糖。蛋白多糖可以根据其电荷和大小分级分离成许多异质池。分离的主要蛋白聚糖很小，含有硫酸软骨素或硫酸皮肤素，最有可能对应于核心蛋白聚糖和双糖蛋白聚糖。还鉴定了具有类似于多功能蛋白聚糖和多配体蛋白聚糖的特征的其他分子。
The proteoglycans synthesized by fibroblasts derived from healthy human adult dental pulps have been isolated and characterized on the basis of their glycosaminoglycan content, molecular size and charge. The proteoglycans were identified by their labelling with [35S] sulphate and susceptibility to digestion by papain. The sulphated glycosaminoglycans associated with the proteoglycans were identified following specific enzymatic and chemical degradations as chondroitin sulphate, dermatan sulphate and heparan sulphate. Dermatan sulphate and chondroitin sulphate and heparan sulphate were the principal glycosaminoglycans associated with the cell layers. The proteoglycans could be fractionated on the basis of their charge and size into a number of heterogeneous pools. The principal proteoglycans isolated were small and contained either chondroitin sulphate or dermatan sulphate and most likely correspond to decorin and biglycan. Other molecules with features similar to versican and syndecan were also identified.