A pleiotropic drug resistance transporter in Nicotiana tabacum is involved in defense against the herbivore Manduca sexta
A pleiotropic drug resistance transporter in Nicotiana tabacum is involved in defense against the herbivore Manduca sexta
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DOI：
10.1111/j.1365-313x.2012.05108.x
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发表时间：
2012-12-01
期刊：
影响因子：
7.2
通讯作者：
Boutry, Marc
中科院分区：
文献类型：
--
作者：
Bienert, Manuela D.;Siegmund, Stephanie E. G.;Boutry, Marc
多效耐药转运蛋白（PDR）是一类属于ATP结合盒（ABC）转运蛋白ABCG亚家族的膜蛋白。有明确的证据表明，植物ABC转运蛋白参与了对真菌和细菌病原体的抗性，但不参与对昆虫或草食动物攻击的生物胁迫反应。在这里，我们描述了一个PDR转运蛋白，ABCG 5/PDR 5，从烟草。GFP融合和亚细胞分级分离研究显示ABCG 5/PDR 5定位于质膜。在ABCG 5/PDR 5转录启动子控制下表达GUS报告基因的转基因植物的染色和野生型植物的免疫印迹显示，在标准生长条件下，ABCG 5/PDR 5在根、茎和花中高度表达，但在叶中仅以边缘水平表达。有趣的是，ABCG 5/PDR 5表达诱导茉莉酸甲酯，创伤，病原体浸润，或草食性的烟草。为了阐明ABCG 5/PDR 5的生理作用，N.获得ABCG 5/PDR 5表达沉默的烟草植物。在标准条件下未观察到表型改变。然而，观察到对真菌尖孢镰刀菌的敏感性略有增加。一个更强的影响，观察到有关草食性：沉默的植物允许更好的增长和更快的发展M。与野生型植物相比，Sexta幼虫的生长速度更快，表明这种PDR转运蛋白参与了对M.六齿草食动物
Pleiotropic drug resistance (PDR) transporters are a group of membrane proteins belonging to the ABCG sub-family of ATP binding cassette (ABC) transporters. There is clear evidence for the involvement of plant ABC transporters in resistance to fungal and bacterial pathogens, but not in the biotic stress response to insect or herbivore attack. Here, we describe a PDR transporter, ABCG5/PDR5, from Nicotiana tabacum. GFP fusion and subcellular fractionation studies revealed that ABCG5/PDR5 is localized to the plasma membrane. Staining of transgenic plants expressing the GUS reporter gene under the control of the ABCG5/PDR5 transcription promoter and immunoblotting of wild-type plants showed that, under standard growth conditions, ABCG5/PDR5 is highly expressed in roots, stems and flowers, but is only expressed at marginal levels in leaves. Interestingly, ABCG5/PDR5 expression is induced in leaves by methyl jasmonate, wounding, pathogen infiltration, or herbivory by Manduca sexta. To address the physiological role of ABCG5/PDR5, N. tabacum plants silenced for the expression of ABCG5/PDR5 were obtained. No phenotypic modification was observed under standard conditions. However, a small increase in susceptibility to the fungus Fusarium oxysporum was observed. A stronger effect was observed in relation to herbivory: silenced plants allowed better growth and faster development of M. sexta larvae than wild-type plants, indicating an involvement of this PDR transporter in resistance to M. sexta herbivory.