INFLUENCE OF CHANGES IN THE NURSE CELL SYSTEM (SYNCYTIUM) ON SEX DETERMINATION AND DEVELOPMENT OF THE CYST NEMATODE HETERODERA-SCHACHTII - TOTAL AMOUNTS OF PROTEINS AND AMINO-ACIDS
INFLUENCE OF CHANGES IN THE NURSE CELL SYSTEM (SYNCYTIUM) ON SEX DETERMINATION AND DEVELOPMENT OF THE CYST NEMATODE HETERODERA-SCHACHTII - TOTAL AMOUNTS OF PROTEINS AND AMINO-ACIDS
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DOI：
10.1094/phyto-81-70
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发表时间：
1991-01-01
期刊：
影响因子：
3.2
通讯作者：
WYSS, U
中科院分区：
文献类型：
--
作者：
GRUNDLER, F;BETKA, M;WYSS, U
研究了芜菁幼苗根系的营养状况。西尔弗斯里斯湾通过对甜菜胞囊线虫（Heterodera schachtii）‘Stielmus’的性别鉴定，研究了甜菜胞囊线虫的性别决定是受环境因素还是遗传因素的控制。 在无菌条件下，将单个J2幼虫加入到水琼脂滴中的发芽根中。 通过去除根尖和芽将一半的测试植物去头。 加入线虫两天后，将含有矿物质和各种浓度蔗糖的营养液倒在琼脂滴上。 接种后12天，线虫的发展，在10个处理组合进行了检查，并在合胞体，相邻的根段，和未接种的控制根的蛋白质和氨基酸的总量进行了测定与敏感的微量法。 7种处理组合支持和3种抑制雌性发育。 实验提供的证据表明，在有利的条件下，大多数青少年发展为女性。 在抑制少年发展的治疗，青少年仍然在J2或J3阶段的比例高，导致营养供应的变化。 合胞体和对照根段中蛋白质和氨基酸总量的变化对雌性发育没有明显影响。
The nutrition of seedling roots of Brassica rapa var. silverstris f. campestris 'Stielmus' was manipulated to test whether sex determination in the beet cyst nematode, Heterodera schachtii, is controlled by environmental or genetic factors. Single J2 juveniles were added to germinated roots in water agar drops under aseptic conditions. Half of the test plants were decapitated by removing root tips and shoots. Two days after adding the nematodes, nutrient solution containing minerals and various concentrations of sucrose was poured over the agar drops. Twelve days after inoculation, nematode development in the 10 treatment combinations was examined, and the total amounts of protein and amino acids in the syncytia, adjacent root segments, and uninoculated control roots were determined with sensitive micromethods. Seven treatment combinations supported and three inhibited female development. The experiments provided evidence that under favorable conditions, most juveniles develop as females. In treatments that inhibited juvenile development, the high proportion of juveniles still at the J2 or J3 stage resulted from changes in the nutrient supply. Changes in the total amount of proteins and amino acids in the syncytia, as well as in the control root segments, did not obviously influence female development.