Effects of disialoganglioside GD3 on the mitochondrial membrane potential
Effects of disialoganglioside GD3 on the mitochondrial membrane potential
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DOI：
10.1016/j.febslet.2005.04.054
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发表时间：
2005-06-06
期刊：
影响因子：
3.5
通讯作者：
Ohta, Y
中科院分区：
文献类型：
--
作者：
Higuchi, Y;Miura, T;Ohta, Y
GD3是细胞凋亡信号传导的细胞内介质。虽然已知GD3直接作用于线粒体，但单个线粒体对GD3的动态响应仍有待阐明。在目前的研究中，在GD3或其类似物的存在下观察到单个线粒体的膜电位。在这里，我们报告（1）GD3特异性诱导内膜的逐渐去极化的机制，不同于渗透性转换，（2）GD3诱导的去极化被环孢菌素A抑制。这些结果表明，GD3通过与渗透性转变不同但相关的机制使线粒体去极化。(c)2005年欧洲生物化学学会联合会。Elsevier B.V.出版，保留所有权利。
GD3 is an intracellular mediator of apoptotic signaling. Although GD3 is known to directly act on mitochondria, the dynamic responses of individual mitochondria to GD3 remain to be elucidated. In the current study, the membrane potential of single mitochondria is observed in the presence of GD3 or its analogues. Here, we report that (1) GD3 specifically induces gradual depolarizations of the inner membrane by a mechanism that differs from the permeability transition, and (2) the GD3-induced depolarizations are suppressed by cyclosporin A. These results suggest that GD3 depolarizes mitochondria by a mechanism distinct from but relevant to the permeability transition. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.