Asbestos-induced alterations of human lymphoid cell mitogenic responses.
Asbestos-induced alterations of human lymphoid cell mitogenic responses.
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石棉诱导的人淋巴细胞有丝分裂反应的改变。
DOI：
10.1016/0013-9351(83)90214-1
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发表时间：
1983
影响因子：
8.3
通讯作者：
Salvaggio,JE
中科院分区：
文献类型：
--
作者：
Bozelka,BE;Gaumer,HR;Nordberg,J;Salvaggio,JE
通过促有丝分裂测定，我们研究了两种石棉（铁石棉和温石棉）纤维对体外淋巴细胞功能的短期影响。这些带相反电荷的纤维对有丝分裂产生不同的改变。培养基中添加 6 μg 温石棉后，伴刀豆球蛋白-A (Con-A) 和商陆有丝分裂原刺激的人外周血单核细胞 (PBMN) 的胚芽细胞反应显着增加。铁石棉纤维在所有测试中均被证明具有抑制作用。当 PBMN 中的单核细胞耗尽时，其余细胞中与石棉相关的 Con-A 反应性变化没有变化。然而，在植物血凝素（PHA）培养物中添加温石棉会导致有丝分裂反应显着增加。当 PBMN 富集 T 淋巴细胞并再次与有丝分裂原和纤维一起培养时，Con-A 反应仍然显示出与温石棉的显着增强。与完整的 PBMN 群体相比，PHA 诱导的这些 T 富集淋巴细胞的胚细胞发生显着升高。这些实验表明，在培养中相对较短的暴露时间后，石棉可以引起 PBMN 功能完整性的显着变化。
Using mitogenic assays, we have investigated the short term effects of two asbestos (amosite and chrysotile) fibers on lymphocyte functionsin vitro. These oppositely charged fibers produced different alterations in mitogenesis. The blastogenic responses of concanavalin-A (Con-A) and pokeweed mitogen stimulated human peripheral blood mononuclear cells (PBMN) were significantly increased by the inclusion of 6 μg of chrysotile to the culture media. Amosite fibers proved to be inhibitory in all tests. When PBMN were depleted of monocytes, asbestos-related alterations of Con-A responsiveness were unchanged among the remaining cells. However, the addition of chrysotile to phytohemagglutinin (PHA) cultures resulted in a significant increase of the mitogenic response. When PBMN were enriched for T lymphocytes, and again cultured with the mitogens and fibers, the Con-A response still displayed impressive enhancement with chrysotile. In contrast to an intact PBMN population, PHA-induced blastogenesis among these T-enriched lymphocytes was significantly elevated. These experiments demonstrate that asbestos can induce significant changes in the functional integrity of PBMN following a relatively short exposure time in culture.