ASYMMETRIC OXIDOREDUCTIONS CATALYZED BY ALCOHOL-DEHYDROGENASE IN ORGANIC-SOLVENTS
ASYMMETRIC OXIDOREDUCTIONS CATALYZED BY ALCOHOL-DEHYDROGENASE IN ORGANIC-SOLVENTS
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DOI：
10.1021/ja00281a044
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发表时间：
1986-10-15
影响因子：
15
通讯作者：
KLIBANOV, AM
中科院分区：
文献类型：
--
作者：
GRUNWALD, J;WIRZ, B;KLIBANOV, AM
开发了一种在有机溶剂中使用醇脱氢酶（和其他NAD+/NADH依赖性酶）作为催化剂的方法。将酶和辅因子沉积在玻璃珠的表面上，然后将玻璃珠悬浮在含有底物的与水不混溶的有机溶剂中。NADH和NAD+都有效地再生，在这样的系统与醇脱氢酶催化的氧化乙醇和还原异丁醛，分别辅因子营业额为105至大于106已获得。利用马肝醇脱氢酶在异丙醚中催化的不对称氧化还原反应，以1 - 10 mmol的规模制备了光学活性（ee为95 - 100%）的醇和酮。
A methodology is developed for the use of alcohol dehydrogenase (and other NAD+/NADH-dependent enzymes) as catalysts in organic solvents. The enzyme and the cofactor are deposited onto the surface of glass beads which are then suspended in a water-immiscible organic solvent containing the substrate. Both NADH and NAD+ are efficiently regenerated in such a system with alcohol dehydrogenase-catalyzed oxidation of ethanol and reduction of isobutyraldehyde, respectively; cofactor turnover numbers of 105 to greater than 106 have been obtained. With use of asymmetric oxidoreductions catalyzed by horse liver alcohol dehydrogenase in isopropyl ether, optically active (ee of 95 to 100%) alcohols and ketones have been prepared on a 1 to 10 mmol scale.