Efficient inhibition of foot-and-mouth disease virus replication in vitro by artificial microRNA targeting 3D polymerase
Efficient inhibition of foot-and-mouth disease virus replication in vitro by artificial microRNA targeting 3D polymerase
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DOI：
10.4149/av_2019_407
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发表时间：
2019-01-01
期刊：
影响因子：
1.7
通讯作者：
Venkataramanan, R.
中科院分区：
文献类型：
--
作者：
Basagoudanavar, S. H.;Ranjitha, H. B.;Venkataramanan, R.
口蹄疫（FMD）是一种毁灭性的偶蹄牲畜急性病毒性疾病。在各种治疗控制措施中，RNA干扰（RNAi）是正在探索的抑制FMD病毒复制和传播的方法之一。 RNAi 通过短发夹 RNA 或人工 microRNA (amiRNA) 实现。 amiRNA 作为抗病毒药物、靶向病毒基因组保守区域的用途变得越来越重要。然而，miRNA在体内的递送仍然是一个挑战。在本研究中，通过生成表达针对FMD病毒（FMDV）基因组的三个功能区域（IRES、3B3和3D）的amiRNA的稳定细胞系，研究了amiRNA在允许的细胞培养系统中阻止FMD病毒复制的功效。结果表明，靶向3D聚合酶的amiRNA相对更有效。然而，针对这三个区域的多个 microRNA 的表达并没有表现出相加效应。数据表明，3D 特异性 miRNA 是开发针对 FMDV 感染的新型抗病毒措施的潜在有效策略。
Foot-and-mouth disease (FMD) is a devastating acute viral disease of livestock with cloven hooves. Among various therapeutic control measures, RNA interference (RNAi) is one of the methods being explored to inhibit FMD virus replication and spread. The RNAi is achieved by short hairpin RNAs or artificial microRNAs (amiRNAs). Utility of amiRNAs as antiviral, targeting conserved regions of the viral genome is gaining importance. However, delivery of miRNA in vivo is still a challenge. In this study, the efficacy of amiRNAs in preventing FMD virus replication in a permissive cell culture system was investigated, by generating stable cell lines expressing amiRNAs targeting three functional regions of the FMD virus (FMDV) genome (IRES, 3B3 and 3D). The results showed that amiRNA targeting 3D polymerase is relatively more efficient. However, expression of multiple microRNAs targeting the three regions did not exhibit additive effect. The data suggest that 3D specific miRNA is a potential valid strategy in developing novel antiviral measures against FMDV infection.