Simple Liquid Chromatography-Mass Spectrometry Method for Quantification of Major Free Oligosaccharides in Bovine Milk
Simple Liquid Chromatography-Mass Spectrometry Method for Quantification of Major Free Oligosaccharides in Bovine Milk
复制标题
DOI：
10.1021/jf5037849
复制
发表时间：
2014-11-26
影响因子：
6.1
通讯作者：
Rochfort, Simone
中科院分区：
文献类型：
--
作者：
Liu, Zhiqian;Moate, Peter;Rochfort, Simone
游离低聚糖（OS）是乳中重要的功能性成分，难以定量。本文介绍了一种简便的牛乳中主要游离OS的定量分析方法。脱脂步骤后，通过超滤进行蛋白质消除。采用亲水相互作用液相色谱（HILIC）分离OS，用Orbitrap质量分析仪在负离子模式下检测OS。该方法对所有代表性OS均具有灵敏度[检测限（LOD）]，
Free oligosaccharides (OS) are a significant functional component of milk that are difficult to quantitate. A simple method for quantitative analysis of the major free OS in bovine milk is described. Following a defatting step, protein elimination was performed by ultrafiltration. OS were separated by hydrophilic interaction liquid chromatography (HILIC) and detected by an Orbitrap mass analyzer in negative mode. The method is sensitive [with a limit of detection (LOD) for all representative OS of