Ordered recruitment of transcription and chromatin remodeling factors to a cell cycle- and developmentally regulated promoter (Publication with Expression of Concern)
Ordered recruitment of transcription and chromatin remodeling factors to a cell cycle- and developmentally regulated promoter (Publication with Expression of Concern)
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DOI：
10.1016/s0092-8674(00)80740-0
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发表时间：
1999-04-30
期刊：
影响因子：
64.5
通讯作者：
Nasmyth, K
中科院分区：
文献类型：
--
作者：
Cosma, MP;Tanaka, TU;Nasmyth, K
真核生物中的基因激活需要染色质重塑复合物如Swi/Snf和组蛋白乙酰化酶如佐贺。这些因子如何被募集到启动子中尚不清楚。使用CHIP，我们测量招聘Swi/Snf，佐贺，阻遏物Ash 1 p，和转录因子Swi 5 p和SBF HO核酸内切酶启动子的细胞通过酵母细胞周期的进展。Swi 5 p在分裂后期进入细胞核后，可募集Swi/Snf，后者再募集佐贺。这两个因素促进了SBF的结合。Ash 1 p仅在子细胞核中积累，在Swi 5 p后不久与HO结合，并中止Swi/Snf、佐贺和SBF的募集。Swi/Snf和佐贺随后在HO的持久性是自我维持的，并构成了HO与Swi 5 p的短暂相互作用的“表观遗传记忆”。
Gene activation in eukaryotes requires chromatin remodeling complexes like Swi/Snf and histone acetylases like SAGA. How these factors are recruited to promoters is not yet understood. Using CHIP, we measured recruitment of Swi/Snf, SAGA, the repressor Ash1p, and transcription factors Swi5p and SBF to the HO endonuclease promoter as cells progress through the yeast cell cycle. Swi5p's entry into nuclei at the end of anaphase recruits Swi/Snf, which then recruits SAGA. These two factors then facilitate SBF's binding. Ash1p, which only accumulates in daughter cell nuclei, binds to HO soon after Swi5p and aborts recruitment of Swi/Snf, SAGA, and SBF. Swi5p remains at HO for only 5 min. Swi/Snf's and SAGA's subsequent persistence at HO is self sustaining and constitutes an "epigenetic memory" of HO's transient interaction with Swi5p.