Direct effect of interleukin 2 on the differentiation of human B cells which have not been preactivated in vitro.
Direct effect of interleukin 2 on the differentiation of human B cells which have not been preactivated in vitro.
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白细胞介素2对体外未预激活的人B细胞的分化有直接影响。
DOI：
10.1002/eji.1830151102
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发表时间：
1985
影响因子：
5.4
通讯作者：
Fauci,AS
中科院分区：
文献类型：
--
作者：
LethiBich-Thuy;Fauci,AS
重组白细胞介素2（IL 2）可诱导人扁桃体B细胞分化，而不需要体外预激活信号。这些细胞悬液在培养开始和终止时均不含可检测的T细胞、单核细胞或自然杀伤细胞，并且对T细胞依赖性多克隆B细胞活化剂美洲商陆有丝分裂原无应答。在不存在IL 2的情况下，在培养开始和结束时，它们含有非常少的Tac抗原携带细胞（小于2.5%）。在存在IL 2的情况下，仅观察到Tac抗原携带细胞数量的极轻微增加。观察到的分化并不是由于污染T细胞的影响，因为故意污染高达5% T细胞的培养物并不比不添加T细胞的培养物含有更多的IgM、IgG或伊加。此外，当T细胞以IT细胞/9个B细胞的比例存在时，IgM的产生减少。综上所述，这些数据支持以下假设：（a）B细胞对IL 2的反应性分化可能在没有体外预活化的情况下发生，在这种情况下，与B细胞表面Tac抗原的表达无关;（B）它不是由于极少量的T细胞的辅助效应可能污染B细胞悬浮液;和（c）它很可能是由IL 2对B细胞本身的直接作用引起的。这些数据还指出，在IL 2存在的情况下，T细胞可能对IL 2诱导的B细胞分化产生抑制作用，而不是提供辅助信号。
Unfractionated as well as small and large human tonsillar B cells were found to differentiate in the presence of recombinant interleukin 2 (IL 2) without requiring anin vitropreactivation signal. These cell suspensions did not contain detectable T cells, monocytes or natural killer cells both at the initiation and the termination of cultures, and did not respond to the T cell‐dependent polyclonal B cell activator pokeweed mitogen. They contained very few Tac antigen‐bearing cells (less than 2.5%) at the beginning and at the termination of the cultures in the absence of IL 2. In the presence of IL 2, only a minimal increase in the number of Tac antigen‐bearing cells was noted. The observed differentiation was not due to the effects of contaminating T cells, since cultures purposely contaminated with up to 5% of T cells did not contain greater amounts of IgM, IgG or IgA than cultures without T cells added back. Furthermore, when T cells were present at a ratio of IT cell per 9 B cells, the production of IgM was decreased. Taken together, these data support the hypothesis that (a) B cell differentiation in response to IL 2 may occur in the absence ofin vitropreactivation, and in this case cannot be related to the expression of Tac antigen on the B cell surface; (b) it is not due to helper effects of very low numbers of T cells which might contaminate the B cell suspensions; and (c) it most likely results from a direct effect of IL 2 on B cells themselves. The data also point out the fact that, in the presence of IL 2, T cells may trigger inhibitory effects on IL2‐induced B cell differentiation rather than provide helper signals.