The temporal requirement for endothelin receptor-B signalling during neural crest development
The temporal requirement for endothelin receptor-B signalling during neural crest development
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DOI：
10.1038/990040
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发表时间：
1999-12-02
期刊：
影响因子：
64.8
通讯作者：
Tilghman, SM
中科院分区：
文献类型：
--
作者：
Shin, MK;Levorse, JM;Tilghman, SM
内皮素受体B （EDNRB）是一种具有7个跨膜结构域的g蛋白偶联受体，是黑素细胞和肠神经元发育所必需的。Ednrb基因零突变纯合子的小鼠几乎完全是白色的，并且在幼年时死于巨结肠癌。为了确定胚胎发生过程中何时需要Ednrb信号传导，我们利用四环素诱导系统产生了内源性Ednrb位点受四环素依赖的反激活因子tTa或rtTA控制的小鼠菌株。通过使用该系统在胚胎发生的不同阶段表达Ednrb，我们已经确定在胚胎10天至12.5天的神经嵴发育期间需要Ednrb。此外，我们的结果表明，EDNRB是黑素母细胞和肠神经母细胞迁移所必需的。
Endothelin receptor B (EDNRB) is a G-protein-coupled receptor with seven transmembrane domains which is required for the development of melanocytes and enteric neurons. Mice that are homozygous for a null mutation in the Ednrb gene are almost completely white and die as juveniles from megacolon, To determine when EDNRB signalling is required during embryogenesis, we have exploited the tetracycline-inducible system to generate strains of mice in which the endogenous Ednrb locus is under the control of the tetracycline-dependant transactivators tTa or rtTA. By using this system to express Ednrb at different stages of embryogenesis, we have determined that EDNRB is required during a restricted period of neural crest development between embryonic days 10 and 12.5. Moreover, our results imply that EDNRB is required for the migration of both melanoblasts and enteric neuroblasts.