Analysis of porcine OSBPL5 gene allelic expression in skeletal muscle and association of a single-nucleotide polymorphism in the coding region with production traits
Analysis of porcine OSBPL5 gene allelic expression in skeletal muscle and association of a single-nucleotide polymorphism in the coding region with production traits
复制标题
猪骨骼肌OSBPL5基因等位基因表达分析及其编码区单核苷酸多态性与生产性状的关联
DOI：
10.1139/cjas-2018-0253
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发表时间：
2019-12-01
影响因子：
1
通讯作者：
Jiang，Yunliang
中科院分区：
文献类型：
--
作者：
Wang，Meng;Wei，Deli;Jiang，Yunliang
摘要：表现出等位基因表达不平衡的基因和印记基因在胚胎存活和出生后生长调节中起重要作用。本研究对猪氧化固醇结合蛋白相关基因5（OSBPL5）进行了检测，并在莱芜猪和长白猪的比较中发现了OSBPL5基因编码区2140G>A突变（rs318687202）。通过基于单核苷酸多态性（SNP）的等位基因特异性表达分析，对OSBPL 5基因在成年猪和新生猪骨骼肌中的印迹状态进行了研究。结果表明，OSBPL 5在成年人骨骼肌中具有父系印记，但在新生儿骨骼肌中以母系印记为主。分析了2140G>A SNP在4个猪群体中的分布，杜洛克猪和大普连猪群体中GG基因型占优势，军牧1号和莱芜猪群体中AG基因型占优势。GG基因型猪的窝产仔数和炮筒骨周长显著大于AA基因型猪，但平均日增重较低。总之，我们确定了成年猪和新生猪之间OSBPL5等位基因表达的差异，并确定了其编码区中与生产性状相关的SNP。
Abstract: Genes that exhibit allelic expression imbalance and imprinted genes play important roles in the survival of the embryo and postnatal growth regulation. In this study, the porcine oxysterol-binding protein-related 5 (OSBPL5) gene was examined, and the 2140G>A mutation (rs318687202) was found in its coding region by a comparison of Laiwu and Landrace pigs. By allele-specific expression analysis based on a specific single-nucleotide polymorphism (SNP), the imprinting status of OSBPL5 gene in skeletal muscle from both neonate and adult pigs was determined. The results showed that the OSBPL5 was paternally imprinted in skeletal muscle from adults but biallelically expressed with predominantly maternal imprinting in neonates. The distribution of the 2140G>A SNP in four pig populations was analyzed, which showed that GG genotype was dominant in Duroc and Dapulian populations, whereas the AG genotype was dominant in Junmu-1 and Laiwu populations. Pigs with the GG genotype had significantly larger litters and greater cannon bone circumferences but a lower average daily gain than pigs with the AA genotype. In conclusion, we determined the difference in the allelic expression of OSBPL5 between adult and neonate pigs and identified an SNP in its coding region that is associated with production traits.