Germ-Free Mice Model for Studying Host-Microbial Interactions

Germ-Free Mice Model for Studying Host-Microbial Interactions
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DOI:
10.1007/978-1-4939-3661-8_8
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发表时间:
2016-01-01
期刊:
MOUSE MODELS FOR DRUG DISCOVERY: METHODS AND PROTOCOLS, 2ND EDITION
影响因子:
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通讯作者:
Kashyap, Purna C.
Kashyap, Purna C.
中科院分区:
其他
文献类型:
--
作者:
Bhattarai, Yogesh;Kashyap, Purna C.

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无菌小鼠是研究健康和疾病中宿主-微生物相互作用的一个相关模型系统。在本章中,我们通过对现有文献的回顾,强调了利用GF小鼠模型研究宿主-微生物相互作用在肥胖、免疫发育和胃肠道生理中的重要性。此外,我们还提供了一个简单的方案,如何建立一个无菌设施,以适当地维持和评估小鼠菌落的GF状态。
Germ-free (GF) mice are a relevant model system to study host-microbial interactions in health and disease. In this chapter, we underscore the importance of using GF mice model to study host-microbial interactions in obesity, immune development and gastrointestinal physiology by reviewing current literature. Furthermore, we also provide a brief protocol on how to setup a gnotobiotic facility in order to properly maintain and assess GF status in mice colonies.