The SNARE motif of synaptobrevin exhibits an aqueous-interfacial partitioning that is modulated by membrane curvature.
The SNARE motif of synaptobrevin exhibits an aqueous-interfacial partitioning that is modulated by membrane curvature.
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DOI：
10.1021/bi401638u
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发表时间：
2014-03-11
期刊：
影响因子：
2.9
通讯作者：
Cafiso, David S.
中科院分区：
文献类型：
--
作者：
Liang, Binyong;Dawidowski, Damian;Ellena, Jeffrey F.;Tamm, Lukas K.;Cafiso, David S.
全长囊泡陷阱，synaptobrevin，的结构和界面协会进行了比较，在四个不同的脂质环境中使用核磁共振和电子顺磁共振光谱。在胶束中，SNARE基序的片段是螺旋状的，并与界面相关联。然而，螺旋和界面协会的分数减少小泡突触蛋白从胶束双分子层的环境中移动，表明界面协会的趋势是敏感的膜曲率。在双分子层中，SNARE基序的小突触泡蛋白瞬时与脂质界面，胶束中的螺旋区域在双分子层和双分子层中进行构象和环境交换。这项工作表明，突触泡蛋白的SNARE基序具有在SNARE组装之前形成螺旋并与膜界面交换的显着倾向。这种短暂的界面结合及其对膜曲率的敏感性可能在调节膜融合的SNARE识别事件中发挥作用。
The structure and interfacial association of the full-length vesicle SNARE, synaptobrevin, were compared in four different lipid environments using nuclear magnetic resonance and electron paramagnetic resonance spectroscopy. In micelles, segments of the SNARE motif are helical and associated with the interface. However, the fraction of helix and interfacial association decreases as synaptobrevin is moved from micelle to bicelle to bilayer environments, indicating that the tendency toward interfacial association is sensitive to membrane curvature. In bilayers, the SNARE motif of synaptobrevin transiently associates with the lipid interface, and regions that are helical in micelles are in conformational and environmental exchange in bicelles and bilayers. This work demonstrates that the SNARE motif of synaptobrevin has a significant propensity to form a helix and exchange with the membrane interface prior to SNARE assembly. This transient interfacial association and its sensitivity to membrane curvature are likely to play a role in SNARE recognition events that regulate membrane fusion.
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