Enhancement of facultative anaerobic denitrifying communities by oxygen release from roots of the macrophyte in constructed wetlands
Enhancement of facultative anaerobic denitrifying communities by oxygen release from roots of the macrophyte in constructed wetlands
复制标题
通过人工湿地中大型植物根部释放氧气来增强兼性厌氧反硝化群落
DOI：
10.1016/j.jenvman.2019.05.136
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发表时间：
2019
影响因子：
8.7
通讯作者：
Zhuang Xuliang
中科院分区：
文献类型：
--
作者：
Sun Haishu;Xu Shengjun;Wu Shanghua;Wang Rui;Zhuang Guoqiang;Bai Zhihui;Deng Ye;Zhuang Xuliang
湿地植物的径向氧损失(ROL)是影响人工湿地(CWS)脱氮效率和微生物活性的重要因素。然而，不同生态位中微生物群落对ROL的响应很少被研究。本研究旨在通过种植肉豆蔻菌的表面流CW处理高浓度养猪场废水，揭示ROL与沉积物、水和根面微生物响应之间的联系。Ti3+-柠檬酸盐比色法表明，该方法具有较高的灵敏度。水生生物为湿地物种，其ROL值为0.019 mg/h/株。通过定量聚合酶链式反应和微生物16S rRNA基因的高通量测序，结果表明：在3个生态位中，根面兼性厌氧反硝化菌的丰度最高，水体(5~10 cm)中兼性厌氧反硝化菌的丰度最低，底泥中的兼性厌氧反硝化菌最少，不动杆菌在水体中具有丰富的优势。根平面中的反硝化细菌主要以富含假单胞菌、气单胞菌和不动杆菌为主。氧源和耗氧量的理论计算表明，水的再曝气应支持好氧层(0~5 cm)的硝化需氧量，ROL可促进兼性厌氧反硝化菌在根面和水体中的生长(5~10 cm)，从而实现CW系统的反硝化。
The radial oxygen loss (ROL) of wetland plants is a crucial factor that can influence the efficiency required for nitrogen (N) removal and microbial activities responsible for N removal in constructed wetlands (CWs). However, the shift of microbial community in different niches in response to ROL has been rarely studied. This study aims to unravel the link between the ROL and microbial response in sediment, water and rhizoplane by a surface flow CW planted withMyriophyllum aquaticumfor treating high-strength swine wastewater. Ti3+-citrate colorimetric method demonstrated thatM. aquaticumwas a wetland species with a ROL of 0.019 mg/h/plant. Using quantitative polymerase chain reactions (qPCR) and high-throughput sequencing of microbial 16S rRNA gene, we demonstrated that the abundance of facultative anaerobic denitrifiers in the rhizoplane was the most of the three niches, that in the water (5–10 cm) was the less and that in the sediment was the least.Acinetobacterwas enriched and dominated amongst denitrifiers in the water. Denitrifiers in the rhizoplane were mainly dominated by enrichedPseudomonas,Aeromonas, andAcinetobacter. The theoretical calculation of oxygen sources and consumptions indicated that water reaeration should support the oxygen demands for nitrification in the aerobic layer (0–5 cm), and the ROL could stimulate the growth of facultative anaerobic denitrifiers in the rhizoplane and water (5–10 cm) to achieve denitrification within CW systems.