Use of antibodies to probe membrane glycoproteins associated with drug-resistant J774.2 cells.
Use of antibodies to probe membrane glycoproteins associated with drug-resistant J774.2 cells.
复制标题
使用抗体探测与耐药 J774.2 细胞相关的膜糖蛋白。
DOI：
10.1016/0006-291x(87)91415-x
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发表时间：
1987
影响因子：
3.1
通讯作者：
Horwitz,SB
中科院分区：
文献类型：
--
作者：
Zeheb,R;Beittenmiller,HF;Horwitz,SB
小鼠巨噬细胞样细胞系J774的三个耐药亚系在体外选择2个，因为它们能够在高浓度的紫杉醇、长春碱或秋水仙碱中生长。每个都含有一个主要的质膜糖蛋白（130-150 kDa），这是几乎看不到的药物敏感的亲本细胞系。多克隆抗体，提出了对糖蛋白存在于秋水仙碱和长春碱耐药细胞，用于探测三种糖蛋白之间的关系。我们的观察结果表明，来自不同耐药细胞系的糖蛋白有许多共同的结构域，但并不相同。
Three drug-resistant sublines of the murine macrophage-like cell line J774. 2 were selected in vitro for their ability to grow in high concentrations of either taxol, vinblastine, or colchicine. Each contains a major plasma membrane glycoprotein (130–150 kDa), which is barely seen in the drug-sensitive parental cell line. Polyclonal antibodies, raised against the glycoproteins present in the colchicine-and vinblastine-resistant cells, were used to probe for relationships among the three glycoproteins. Our observations suggest that the glycoproteins from the different drug-resistant cell lines share many common domains but are not identical.