Regulation of luteinizing hormone-receptor and follicle-stimulating hormone-receptor messenger ribonucleic acid levels during development in the neonatal mouse ovary
Regulation of luteinizing hormone-receptor and follicle-stimulating hormone-receptor messenger ribonucleic acid levels during development in the neonatal mouse ovary
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DOI：
10.1095/biolreprod57.3.602
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发表时间：
1997-09-01
影响因子：
3.6
通讯作者：
McBride, MW
中科院分区：
文献类型：
--
作者：
OShaughnessy, PJ;McLelland, D;McBride, MW
正常的性腺发育依赖于促性腺激素的刺激，卵巢发育的控制很可能是通过促性腺激素受体的表达来调节的。在本研究中，我们测量了正常小鼠和缺乏循环促性腺激素的性腺功能低下（hpg/hpg）小鼠卵巢发育过程中lh和fsh受体mRNA水平的定量变化。LH和FSH受体的替代转录本的相对丰度也被确定，结果表明，受体的缩短转录本在所有年龄段都很丰富，LH受体的全长转录本直到出生后第5天才被检测到，尽管编码受体胞外结构域的缩短转录本从出生后第5天到第7天就存在。正常动物的lh受体转录物水平显著增加，但hpg/hpg动物的转录物水平没有变化，fsh受体转录物编码受体的所有结构域在出生时低水平可检测到，在第3天和第5天浓度增加，并在第10天达到峰值。在hpg/hpg动物中，fsh受体mRNA水平在第7天之前是正常的，但此后没有增加。这些结果表明卵巢中lh和fsh受体mRNA水平的早期发育与促性腺激素无关。lh受体mRNA水平的进一步发展依赖于促性腺激素，尽管fsh受体mRNA水平继续独立增加，直到早期次生卵泡出现。
Normal gonadal development is dependent upon stimulation by the gonadotropic hormones, and it is likely that control of ovarian development is regulated by expression of gonadotropin receptors, In this study, quantitative changes in LH-and FSH-receptor mRNA levels were measured in the ovary during development in normal mice and in hypogonadal (hpg/hpg) mice, which lack circulating gonadotropins. The relative abundance of alternate transcripts encoding LH and FSH receptors was also determined, Results show that shortened transcripts of the receptors were abundant at all ages, Full-length transcripts of the LH receptor were not detectable until postnatal Day 5 although shortened transcripts encoding the extracellular domain of the receptor were present from birth, Between Days 5 and 7, LH-receptor transcript levels showed a marked increase in normal animals but no change in hpg/hpg animals, FSH-receptor transcripts encoding all domains of the receptor were detectable at low levers at birth, increased in concentration between Days 3 and 5, and peaked at Day 10. In hpg/hpg animals, FSH-receptor mRNA levels were normal up to Day 7 but failed to increase thereafter These results show that early development of both LH-and FSH-receptor mRNA levels in the ovary is gonadotropin-independent. This coincides with early folliculogenesis to the primary stage, Further development of LH-receptor mRNA levels is gonadotropin-dependent although FSH-receptor mRNA levels continue to increase independently until early secondary follicles are present.