DETECTION OF FELINE CORONAVIRUS RNA IN FECES, TISSUES, AND BODY-FLUIDS OF NATURALLY INFECTED CATS BY REVERSE-TRANSCRIPTASE PCR
DETECTION OF FELINE CORONAVIRUS RNA IN FECES, TISSUES, AND BODY-FLUIDS OF NATURALLY INFECTED CATS BY REVERSE-TRANSCRIPTASE PCR
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DOI：
10.1128/jcm.33.3.684-689.1995
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发表时间：
1995-03-01
影响因子：
9.4
通讯作者：
ROTTIER, PJM
中科院分区：
文献类型：
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作者：
HERREWEGH, AAPM;DEGROOT, RJ;ROTTIER, PJM
建立了一种检测猫冠状病毒（FCoV）RNA的巢式逆转录酶PCR（RT-nPCR）方法。RT-nPCR靶向病毒基因组的高度保守的3 '-非翻译区，并将在该领域检测大多数（如果不是全部）猫冠状病毒。用RT-nPCR检测FCoV RNA的血浆样品从实验感染的猫早在接种后2天。FCoV RNA也检测到血清，血浆，或腹水样品14/18猫（78%）自然发生的猫传染性腹膜炎（FIP）。由于存在表面健康的FCoV携带者，因此RT-PCR用于FLP诊断是有限的。这些无症状的猫在粪便中排出病毒，在一些情况下，血浆中也有可检测到的病毒。由于FCoV感染的性质，我们的血浆或血清RT-PCR检测不能用于建立FIP的明确诊断。然而，这种检测确实提供了一种新的手段来识别无症状的FCoV携带者。因此，RT-nPCR将用于在将猫引入无FCoV猫舍之前对其进行筛选。此外，该检测方法为研究FCoV的流行病学提供了重要工具。
A nested reverse transcriptase PCR (RT-nPCR) was developed for the detection of feline coronavirus (FCoV) RNA in the feces, tissues, and body fluids of infected cats. The RT-nPCR was targeted to the highly conserved 3'-untranslated region of the viral genome and will detect most, if not all, feline coronaviruses in the field. With the RT-nPCR FCoV RNA was detected in plasma samples from experimentally infected cats as early as 2 days postinoculation. FCoV RNA was also detected in serum, plasma, or ascitic fluid samples from 14 of 18 cats (78%) with naturally occurring feline infectious peritonitis (FIP). The use of RT-PCR for FLP diagnosis is limited because of the occurrence of apparently healthy FCoV carriers. These asymptomatic cats shed the virus in the feces and, in a number of cases, also had detectable virus in the plasma. Because of the nature of FCoV infections, our RT-PCR assay with plasma or serum cannot be used to establish a definite diagnosis of FIP. However, this assay does provide a new means to identify asymptomatic FCoV carriers. As such, RT-nPCR will be of use to screen cats before their introduction into FCoV-free catteries. Moreover, this assay provides ah important tool to study the epidemiology of FCoV.