A PP2C-1 Allele Underlying a Quantitative Trait Locus Enhances Soybean 100-Seed Weight
A PP2C-1 Allele Underlying a Quantitative Trait Locus Enhances Soybean 100-Seed Weight
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DOI：
10.1016/j.molp.2017.03.006
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发表时间：
2017-05-01
期刊：
影响因子：
27.5
通讯作者：
Zhang, Jin-Song
中科院分区：
文献类型：
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作者：
Lu, Xiang;Xiong, Qing;Zhang, Jin-Song
栽培大豆在驯化过程中可能会丢失一些有用的遗传位点。野生大豆基因的导入可以拓宽大豆的遗传背景，改良大豆的农艺性状。本研究通过对野生大豆ZYD 7与栽培大豆HN 44杂交获得的重组自交系群体进行全基因组测序和种子重数量性状基因座定位，发现野生大豆ZYD 7的磷酸酶2C-1（PP 2C-1）等位基因对种子重/大小的增加有贡献。PP 2C-1可能通过增加珠被细胞的大小和激活种子性状相关基因的子集来实现这一功能。我们发现PP 2C-1与GmBZR 1（拟南芥BZR 1的大豆直向同源物，油菜素类固醇（BR）信号传导的关键转录因子之一）相关，并促进去磷酸化的GmBZR 1的积累。与此相反，PP 2C-2等位基因的变化的几个氨基酸的N-末端没有表现出这种功能。此外，我们发现GmBZR 1可以提高转基因植物的种子重量/大小。通过对栽培大豆种质资源的分析，我们发现40%的受检种质不具有PP 2C-1等位基因，这表明这些种质可以通过引入该等位基因来改良。总之，我们的研究确定了一个精英等位基因PP 2C-1，它可以提高大豆种子的重量和/或大小，并指出，通过分子辅助育种操纵该等位基因可能会增加大豆和其他豆类/作物的产量。
Cultivated soybeans may lose some useful genetic loci during domestication. Introgression of genes from wild soybeans could broaden the genetic background and improve soybean agronomic traits. In this study, through whole-genome sequencing of a recombinant inbred line population derived from a cross between a wild soybean ZYD7 and a cultivated soybean HN44, and mapping of quantitative trait loci for seed weight, we discovered that a phosphatase 2C-1 (PP2C-1) allele from wild soybean ZYD7 contributes to the increase in seed weight/size. PP2C-1 may achieve this function by enhancing cell size of integument and activating a subset of seed trait-related genes. We found that PP2C-1 is associated with GmBZR1, a soybean ortholog of Arabidopsis BZR1, one of key transcription factors in brassinosteroid (BR) signaling, and facilitate accumulation of dephosphorylated GmBZR1. In contrast, the PP2C-2 allele with variations of a few amino acids at the N-terminus did not exhibit this function. Moreover, we showed that GmBZR1 could promote seed weight/size in transgenic plants. Through analysis of cultivated soybean accessions, we found that 40% of the examined accessions do not have the PP2C-1 allele, suggesting that these accessions can be improved by introduction of this allele. Taken together, our study identifies an elite allele PP2C-1, which can enhance seed weight and/or size in soybean, and pinpoints that manipulation of this allele by molecular-assisted breeding may increase production in soybean and other legumes/crops.