Cloning and the nucleotide sequence of rat glutathione S-transferase P cDNA.
Cloning and the nucleotide sequence of rat glutathione S-transferase P cDNA.
复制标题
大鼠谷胱甘肽S-转移酶P cDNA 的克隆和核苷酸序列。
DOI：
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发表时间：
1985
影响因子：
14.9
通讯作者：
M. Muramatsu
中科院分区：
文献类型：
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作者：
Y. Suguoka;T. Kano;A. Okuda;M. Sakai;T. Kitagawa;M. Muramatsu
以2-乙酰氨基芴（AAF）诱发的大鼠肝癌为模型，用合成的谷胱甘肽S-转移酶P亚基（GST P亚基）的部分氨基酸序列为探针，从poly（A）+ RNA构建的肝癌cDNA文库中筛选出一个新的cDNA文库。分析的四个克隆中的一个含有编码该酶亚基的总氨基酸序列的mRNA区域和完整的3 '非编码区。核苷酸序列表明该酶亚基具有209个氨基酸（计算的Mr= 23，307），不同于其它谷胱甘肽S-转移酶亚基，如Ya和Yc。这些蛋白质之间的氨基酸序列的比较表明，谷胱甘肽S-转移酶P亚基基因已经从祖先基因在比Ya和Yc的分离更早的阶段进化而来，并且在这些酶中至少有三个结构域彼此具有相当大的同源性。在化学诱导的肝细胞癌中这种mRNA的大量增加表明在肝癌发生过程中这种基因的特征性去抑制。
A cDNA library prepared from poly(A)+ RNA of 2-acetylaminofluorene (AAF) induced rat hepatocellular carcinoma was screened by synthetic DNA probes deduced from a partial amino acid sequence of glutathione S-transferase P subunit that had been isolated from the tumor by two-dimensional gel electrophoresis. One of the four clones analyzed contained an mRNA region encoding the total amino acid sequence of this enzyme subunit and the complete 3'-noncoding region. The nucleotide sequence indicates that this enzyme subunit has 209 amino acids (calculated Mr=23,307) distinct from other glutathione S-transferase subunits such as Ya and Yc. Comparison of the amino acid sequences between these proteins indicates that glutathione S-transferase P subunit gene has been evolved from the ancestral gene at an earlier stage than the separation of Ya and Yc and that there are at least three domains having a considerable homology with each other in these enzymes. The very large increase of this mRNA in chemically induced hepatocellular carcinoma suggests a characteristic derepression of this gene during hepatocarcinogenesis.