Early-life exposure to per- and polyfluoroalkyl substances and infant gut microbial composition.
Early-life exposure to per- and polyfluoroalkyl substances and infant gut microbial composition.
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DOI：
10.1097/ee9.0000000000000238
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发表时间：
2023-03
影响因子：
3.6
通讯作者：
Romano, Megan E.
中科院分区：
文献类型：
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作者：
Laue, Hannah E.;Moroishi, Yuka;Palys, Thomas J.;Christensen, Brock C.;Criswell, Rachel L.;Peterson, Lisa A.;Huset, Carin A.;Baker, Emily R.;Karagas, Margaret R.;Madan, Juliette C.;Romano, Megan E.
母乳富含必需营养素和免疫激活化合物，但也是全氟烷基和多氟烷基物质（PFAS）等有毒物质的来源。有证据表明，PFAS的免疫相关作用可能部分是由于微生物组的改变。我们的目的是确定牛奶PFAS暴露与婴儿肠道微生物组之间的关联。使用高效液相色谱-串联质谱法对产后约6周的牛奶中的全氟辛烷磺酸（PFOS）和全氟辛酸（PFOA）进行定量。计算摩尔总和（MPFAS）。护理人员在产后6周（n = 116）和/或1年（n = 119）时收集婴儿粪便样本。粪便DNA进行宏基因组测序。我们估计PFAS与物种多样性和相对多度的线性回归。单和多PFAS模型在完整病例分析中调整了潜在混杂因素，并分别对6周和1年微生物组进行了插补缺失协变量数据。我们对敏感人群进行了分层评估。全氟辛烷磺酸和全氟辛酸分别在94%和83%的牛奶样本中检出。全氟辛烷磺酸与纯母乳喂养婴儿6周时的多样性增加相关[全氟辛烷磺酸加倍β = 0.24，（95% CI = 0.03，0.45），P = 0.03]，与非经产母亲所生婴儿6周时的多样性增加相关[β = 0.37（0.06，0.67），P = 0.02]。在多PFAS模型和完整病例中，估计值最强。在1年时，BPFAS与普通拟杆菌相对丰度相关[（β = -2.34%/加倍（-3.63，-1.05），FDR q = 0.099]。PFAS可能会增加婴儿肠道微生物组的多样性，并改变生物相关细菌的相对丰度。其他分析可能会确定相关的健康结果。
Human milk is rich in essential nutrients and immune-activating compounds but is also a source of toxicants including per- and polyfluoroalkyl substances (PFAS). Evidence suggests that immune-related effects of PFAS may, in part, be due to alterations of the microbiome. We aimed to identify the association between milk PFAS exposure and the infant gut microbiome. PFAS [perfluorooctane sulfonic acid (PFOS) and perfluorooctanoate (PFOA)] were quantified in milk from ~6 weeks postpartum using high-performance liquid chromatography with tandem mass spectrometry. A molar sum (ΣPFAS) was calculated. Caregivers collected infant stool samples at 6 weeks (n = 116) and/or 1 year postpartum (n = 119). Stool DNA underwent metagenomic sequencing. We estimated the association of PFAS with diversity and relative abundances of species with linear regression. Single- and multi-PFAS models adjusted for potential confounders in complete case analyses and with imputed missing covariate data for 6-week and 1-year microbiomes separately. We assessed sensitive populations with stratification. PFOS and PFOA were detected in 94% and 83% of milk samples, respectively. PFOS was associated with increased diversity at 6 weeks among infants fed exclusively human milk [β = 0.24 per PFOS doubling, (95% CI = 0.03, 0.45), P = 0.03] and born to primiparous mothers [β = 0.37 (0.06, 0.67), P = 0.02]. Estimates were strongest in multi-PFAS models and among complete cases. ΣPFAS was associated with Bacteroides vulgatus relative abundance at 1 year [(β = –2.34% per doubling (–3.63, –1.05), FDR q = 0.099]. PFAS may increase infant gut microbiome diversity and alter the relative abundance of biologically relevant bacteria. Additional analyses may identify related health outcomes.
影响因子：
8.3
作者：
Laue, Hannah E.;Moroishi, Yuka;Palys, Thomas J.;Jackson, Brian P.;Madan, Juliette C.;Karagas, Margaret R.
通讯作者：
Karagas, Margaret R.