Galectin 1 expression is associated with tumor invasion and metastasis in stage IB to IIA cervical cancer
Galectin 1 expression is associated with tumor invasion and metastasis in stage IB to IIA cervical cancer
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DOI：
10.1016/j.humpath.2012.04.010
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发表时间：
2013-01-01
期刊：
影响因子：
3.3
通讯作者：
Bae, Duk-Soo
中科院分区：
文献类型：
--
作者：
Kim, Ha-Jeong;Do, In-Gu;Bae, Duk-Soo
凝集素1是一种14kd的层粘连蛋白结合凝集素，参与肿瘤的重要生物学机制，包括肿瘤转化、细胞存活、血管生成、细胞增殖和转移。在本研究中，我们探讨了凝集素1在宫颈癌细胞存活和转移中的作用。采用免疫组化方法检测73例福尔马林固定石蜡包埋宫颈癌组织中凝集素1的表达，并与术后复发的临床病理危险因素进行比较。为了评估凝集素1在细胞增殖和侵袭中的作用，我们使用凝集素1小干扰RNA （siRNA）对宫颈癌细胞系（包括HeLa和SiHa细胞）进行了增殖和侵袭试验。免疫组化分析显示，大多数瘤周间质标本中均有凝集素1的表达（72/73;98.6%）。单因素分析显示，Galectin 1表达与宫颈浸润深度（P= 0.015）和淋巴结转移（P= 0.045）显著相关。当根据凝集素1表达分析所有研究患者的无进展生存时，凝集素1表达与无进展生存无关（P= 0.32）。利用小干扰RNA下调凝集素1可抑制HeLa和SiHa细胞的生长和增殖。此外，凝集素1小干扰RNA显著降低了细胞的侵袭能力。我们的研究结果表明，肿瘤周围间质中聚集素1的高表达与宫颈病变的浸润深度和宫颈癌的淋巴结转移有显著的相关性，聚集素1可能在功能上参与了细胞的增殖和浸润。(C) 2013爱思唯尔公司版权所有。
Galectin 1 is a 14-kd laminin-binding lectin involved in important biologic mechanisms of tumors, including neoplastic transformation, cell survival, angiogenesis, cell proliferation, and metastasis. In this study, we investigated the role of galectin 1 in cell survival and metastasis in cervical cancer. The expression of galectin 1 was determined in 73 formalin-fixed, paraffin-embedded cervical cancer tissues using an immunohistochemical method and compared with clinicopathologic risk factors for recurrence after surgery. To evaluate the role of galectin 1 in cell proliferation and invasion, we performed proliferation and invasion assays with galectin 1 small interfering RNA (siRNA) using cervical cancer cell lines, including HeLa and SiHa cells. Immunohistochemical analysis revealed that galectin 1 expression was found in most peritumoral stroma samples (72/73; 98.6%). Galectin 1 expression was significantly correlated with the depth of invasion in the cervix (P=.015) and lymph node metastasis (P=.045) on univariate analysis. When progression-free survival of all of the patients studied was analyzed based upon galectin 1 expression, galectin 1 expression was not correlated with progression-free survival (P=.32). Down-regulation of galectin 1 using small interfering RNA resulted in the inhibition of cell growth and proliferation of HeLa and SiHa cells. Moreover, the ability of cells to invade was significantly reduced by galectin 1 small interfering RNA. Our results revealed that high galectin 1 expression in peritumoral stroma was significantly correlated with depth of invasion in cervical lesions and lymph node metastasis of cervical cancer and that galectin 1 may be functionally involved in cell proliferation and invasion. (C) 2013 Elsevier Inc. All rights reserved.