Selective Portal Vein Embolization and Colorectal Liver Metastases in Rat: A New Experimental Model for Tumor Growth Study
Selective Portal Vein Embolization and Colorectal Liver Metastases in Rat: A New Experimental Model for Tumor Growth Study
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DOI：
10.1016/j.jss.2010.03.047
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发表时间：
2011-12-01
影响因子：
2.2
通讯作者：
Panis, Yves
中科院分区：
文献类型：
--
作者：
Bretagnol, Frederic;Maggiori, Leon;Panis, Yves
背景门静脉栓塞（PVE）已被提议在主要切除术前诱导肝脏肥大。由于对肿瘤生长的影响存在一些担忧，需要进行实验研究，需要可靠的小动物模型。目的是评估大鼠PVE模型的技术可行性，并报告结直肠肝转移（LM）肿瘤的生长。40只大鼠左肝叶注射DHDK 12细胞诱导LM。在第7天，20只大鼠通过剖腹手术进行门静脉造影，允许左侧PVE。20只无PVE大鼠作为对照。所有大鼠在第30天处死。计算肝脏和肿瘤体积。死亡率为20%（n = 8）。PVE在15/19只大鼠中成功（79%）。与对照组大鼠相比，左侧PVE诱导左叶显著萎缩（3.5 +/- 0.8 vs 7.4 +/- 0.9 mm（3），P < 0.0001）和对侧肥大（5.8 +/- 1.1 vs 3.6 +/- 0.7 mm（3），P < 0.0001）。与对照组相比，PVE组左肝LM肿瘤体积显著减小，分别为124.4 ± 95.7 mm（3）和231.1 ± 90.1 mm（3），P = 0.008。PVE在大鼠中可行，成功率为79%。观察到残留肝脏显著肥大和栓塞肝脏萎缩，表明PVE有效。LM肿瘤生长在栓塞的肺叶中显著下降。我们的模型可用于实验研究，评估肿瘤生长和新药对LM的影响，在模拟人类的情况下，部分肝切除术前。(C)2011 Elsevier Inc. All rights reserved.
Background. Portal vein embolization (PVE) has been proposed to induce hypertrophy of liver before major resection. Because there are some concerns about the effect on tumor growth, experimental research is needed, requiring reliable small animal model. The aim was to assess technical feasibility of PVE model in rat and to report colorectal liver metastases (LM) tumor growth.Methods. LM were induced in 40 rats by injecting DHDK12 cells into the left liver lobe. At d 7, a portography was performed through a laparotomy in 20 rats allowing the left PVE. Twenty rats without PVE served as control. All rats were sacrificed at d 30. Liver and tumor volume were calculated.Results. Mortality rate was 20% (n = 8). PVE was successful in 15/19 rats (79%). Compared with control rats, the left PVE induced both significant atrophy of the left lobe (3.5 +/- 0.8 versus 7.4 +/- 0.9 mm(3), P < 0.0001) and contralateral hypertrophy (5.8 +/- 1.1 versus 3.6 +/- 0.7 mm(3), P < 0.0001). LM tumor volume in the left liver was significantly decreased in PVE group compared to control, 124.4 +/- 95.7 mm(3) versus 231.1 +/- 90.1 mm(3), P = 0.008.Conclusion. PVE is feasible in rats with a 79% success rate. Significant hypertrophy of the remnant liver and atrophy of the embolized liver were noted suggesting the efficacy of PVE. LM tumor growth decreased significantly in the embolized lobe. Our model can be used for experimental studies evaluating tumor growth and effects of new drugs against LM in a situation that mimics the human situation before partial hepatectomy. (C) 2011 Elsevier Inc. All rights reserved.