Handling and detection of 0.8 amol of a near-infrared cyanine dye by capillary electrophoresis with laser-induced fluorescence detection.
Handling and detection of 0.8 amol of a near-infrared cyanine dye by capillary electrophoresis with laser-induced fluorescence detection.
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DOI：
10.1016/s0021-9673(02)01441-3
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发表时间：
2002-12
期刊：
影响因子：
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通讯作者：
Changming Yang;O. Shimelis;Xiaojuan Zhou;Guoding Li;C. Bayle;M. Nertz;Hyeran Lee;L. Strekowski,;G. Patonay;F. Couderc;R. Giese
中科院分区：
文献类型：
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作者：
Changming Yang;O. Shimelis;Xiaojuan Zhou;Guoding Li;C. Bayle;M. Nertz;Hyeran Lee;L. Strekowski,;G. Patonay;F. Couderc;R. Giese
We are interested in the detection of DNA adducts and other trace analytes by labeling them with a fluorescent tag followed by use of capillary electrophoresis with laser-induced fluorescence detection (CE–LIF) for high resolution and sensitivity. Towards this goal, here we report the following: (1) synthesis and handling properties of a near-IR, carboxyl-substituted heptamethine cyanine dye; (2) modification of an existing ball lens LIF detector to provide near-LIF detection with excitation at 785 nm for CE; and (3) corresponding handling and detection of as little as 0.8 amol of the dye by enrich-injection of 4.7 μl of 1·10−13mol/l dye in methanol from an 8-μl volume into a corresponding CE–LIF system. The electrolyte for the separation was methanol–40 mmol/l aqueous sodium borate (98:2, v/v). This finding encourages further exploration of the dye by functionalization of its carboxyl group for chemical labeling purposes.
