Three‐dimensional localisation of NANOG, OCT4, and E‐cadherin in porcine pre‐ and peri‐implantation embryos
Three‐dimensional localisation of NANOG, OCT4, and E‐cadherin in porcine pre‐ and peri‐implantation embryos
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DOI：
10.1002/dvdy.22491
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发表时间：
2011-01
影响因子：
2.5
通讯作者：
X. Wolf;P. Serup;P. Hyttel
中科院分区：
文献类型：
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作者：
X. Wolf;P. Serup;P. Hyttel
先前已报道NANOG和OCT 4的表达模式在哺乳动物物种之间显著不同，表明在发育期间具有不同的物种特异性作用。我们研究了NANOG和OCT 4在猪植入前和植入后胚胎中的三维表达模式。NANOG的表达与其他物种中报道的有显著差异。NANOG未检测到内细胞团孵化猪囊胚，但后来出现在上胚层和下胚层的球形囊胚，其中劳伯氏层已解体。在原肠胚形成前，丝状胚胎NANOG定位于小部分上胚层细胞的细胞核，其中E-钙粘蛋白似乎上调，OCT 4下调。后来NANOG被限制在潜在的PGCs。OCT4在内细胞团、上胚层和中胚层中检测到，我们发现，与早期的推测相反，至少在孵化的囊胚中，OCT4的表达类似于小鼠胚胎中的表达模式。发展动力学，2011年。© 2010 Wiley利斯公司
The expression patterns of NANOG and OCT4 have previously been reported to differ markedly between mammalian species indicating distinct species‐specific roles during development. We investigate the three‐dimensional expression pattern of NANOG and OCT4 in porcine pre‐ and peri‐implantation embryos. The expression of NANOG differed remarkably from that reported in other species. NANOG was not detected in the inner cell mass of hatched porcine blastocysts, but later appeared in the epiblast and hypoblast of spherical blastocysts where Rauber's layer had disintegrated. In pre‐gastrulating, filamentous embryos NANOG was localised to nuclei in a minor portion of the epiblast cells in which E‐CADHERIN seemed to be up‐regulated and OCT4 down‐regulated. Later NANOG was restricted to the potential PGCs. OCT4 was detected in inner cell mass, epiblast, and mesoderm, and we found that OCT4 expression, in contrast to earlier speculations, at least in hatched blastocysts, resembles the expression pattern in the mouse embryo. Developmental Dynamics, 2011. © 2010 Wiley‐Liss, Inc.