Isolation and characterization of emerging subgroup J avian leukosis virus associated with hemangioma in egg-type chickens
Isolation and characterization of emerging subgroup J avian leukosis virus associated with hemangioma in egg-type chickens
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DOI：
10.1016/j.vetmic.2011.03.037
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发表时间：
2011-08-05
影响因子：
3.3
通讯作者：
Liao, Ming
中科院分区：
文献类型：
--
作者：
Lai, Hanzhang;Zhang, Henan;Liao, Ming
禽白血病病毒J亚群（ALV-J）于1989年首次分离，主要引起肉型或蛋型鸡的骨髓性白血病（ML）。自2006年以来，已经报道了与ALV-J相关的商业蛋鸡群中血管瘤而非ML的临床病例，但尚不清楚是否已经出现了新的致癌性ALV-J。通过动物实验和原病毒全长基因组序列分析，对商品罗马蛋鸡血管瘤ALV-J分离株SCAU-HN06进行了鉴定。SPF白来角蛋型鸡在孵育第11天感染SCAU-HN06，在22周的试验中，血管瘤总发生率为56%，肾肿瘤总发病率为8%，死亡率为16%。SCAU-HN06分离物大部分基因序列与海兰褐蛋鸡血管瘤分离物JS09GY6具有较高的同源性。SCAU-HN06和JS09GY6的共同特征是先导序列插入19 bp和E元件缺失1个。SCAU-HN06和与血管瘤相关的ALV-J可能是ALV-J和其他禽类逆转录病毒的重组体，它们可能具有相同的祖先。(C) 2011 Elsevier B.V.版权所有
Subgroup J avian leukosis virus (ALV-J), first isolated in 1989, predominantly causes myeloid leukosis (ML) in meat-type or egg-type chicken. Since 2006, the clinical cases of hemangioma rather than ML in commercial layer flocks associated with ALV-J have been reported, but it was still not clear whether the novel oncogenic ALV-J had emerged. We characterized SCAU-HN06 isolate of ALV-J from hemangioma in commercial Roman layers through animal experiment and full-length proviral genome sequence analysis. The SPF white leghorn egg-type chickens infected with SCAU-HN06 in ovo at day 11 of incubation showed an overall incidence of 56% hemangioma and 8% renal tumor throughout the 22-week trial, the mortality rate was 16%. Most genes of SCAU-HN06 isolate showed high nucleotide sequence identity to JS09GY6 which was isolated from Hy-Line Variety Brown layers suffering hemangioma. The 19-bp insertion in leader sequence and one key deletion in E element were the common features of SCAU-HN06 and JS09GY6. SCAU-HN06 and those ALV-Js associated with hemangioma, possibly recombinants of ALV-J and other avian retrovirus, may share the same ancestor. (C) 2011 Elsevier B.V. All rights reserved.