THE CYTOARCHITECTURE OF THE DORSAL COCHLEAR NUCLEUS IN THE 3-MONTH-OLD AND 26-MONTH-OLD C57BL/6 MOUSE - A GOLGI IMPREGNATION STUDY
THE CYTOARCHITECTURE OF THE DORSAL COCHLEAR NUCLEUS IN THE 3-MONTH-OLD AND 26-MONTH-OLD C57BL/6 MOUSE - A GOLGI IMPREGNATION STUDY
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DOI：
10.1002/cne.902110203
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发表时间：
1982-01-01
影响因子：
2.5
通讯作者：
BARUCH, A
中科院分区：
文献类型：
--
作者：
BROWNER, RH;BARUCH, A
比较3和26个月龄大鼠耳蜗背核（DCN）的细胞构筑。老年C57 BL/6小鼠。用Nissl染色和Golgi浸渍材料分析了该小鼠品系CNS中存在的遗传控制的进行性听力损失的影响。DCN可分为表层分子层、中层梭形颗粒层和深层多晶层。分子层（ML）由许多纤维和少数小的卵球形、梭形和颗粒细胞组成。梭形颗粒层（FL）含有大的梭形和许多颗粒细胞。大多数FL梭形细胞的长轴方向垂直于DCN表面，并作为小聚集体或单独存在。车轮细胞与FL梭形细胞相邻。深多形层（PL）含有球形、梭形、颗粒和多极神经元。颗粒细胞形成DCN的背帽。从这个帽，颗粒细胞片将DCN与后腹侧耳蜗核（PVCN）和脑干分开。两个年龄组的内部组织、神经元位置、方向和形态相似。颗粒细胞有4-5个初级树突，静脉曲张，很少或没有树突附属物。FL梭形细胞在两个年龄段表现出不同的树突状形态。在3月龄时有一个或两个精致的初级ML顶树突。年老的老鼠身上长满了穗状的树突棘。基底1或2 PL树突不太精致，很少有树突棘。26个月时FL梭形神经元老年小鼠的树突状细胞排列规则，棘少，短而粗。基底树突有静脉曲张和中断。3个月时的侧手翻神经元老年小鼠有精心制作的ML树突树，树突棘覆盖。26个月后老年小鼠树突有许多静脉曲张和较少的短钝树突棘。老年小鼠的大多极神经元比3个月大的小鼠有更薄的树突和更多的静脉曲张。老组。在这两个年龄组中，多极细胞的树突棘远端有限。大、小球形细胞有2-5个初级树突，树突呈静脉曲张状，很少有高级分支和棘。梭形细胞有1或2个初级树突，很少次级分支，很少或没有刺。在2个年龄组中，球形和梭形细胞中观察到轻微退行性变化。这些包括树突的静脉曲张，中断和一些不规则的胞体表面。耳蜗中存在的退行性变化对有限的DCN神经元群体有显著影响。C57 BL/6小鼠DCN的神经元形态和结构与其他哺乳动物相似。
The cytoarchitecture of the dorsal cochlear nucleus (DCN) was compared in 3- and 26-mo.-old C57BL/6 mice. The effects of genetically controlled progressive hearing loss present in the CNS in this mouse strain were analyzed with Nissl-stained and Golgi-impregnated material. The DCN was divided into the superficial molecular, an intermediate fusiform-granule and the deep polymorphic layers. The molecular layer (ML) consisted of many fibers and a few small ovoid to spherical, fusiform and granule cells. The fusiform-granule layer (FL) contained large fusiform and many granule cells. Most FL fusiform cells were oriented with their long axes perpendicular to the DCN surface and were present as small aggregations or individually. Cartwheel cells were adjacent to the FL fusiform cells. The deep polymorphic layer (PL) contained spherical, fusiform, granule and multipolar neurons. The granule cells formed a dorsal cap of the DCN. From this cap, sheets of granule cells separated the DCN from the posterior ventral cochlear nucleus (PVCN) and from the brainstem. The internal organization, neuronal location, orientation and morphology were similar in both age groups. The granule cells had 4-5 primary dendrites, varicosities and few to no dendritic appendages. The FL fusiform cells displayed different dendritic morphology in the 2 ages. One or two elaborate primary ML apical dendrites in the 3-mo.-old mice were covered with spikelike dendritic spines. The basal 1 or 2 PL dendrites were less elaborate and had few dendrite spines. FL fusiform neurons in 26-mo.-old mice had regular dendritic varicosities and fewer spines which were short and stumpy. Basal dendrites had varicosities and interruptions. Cartwheel neurons in 3-mo.-old mice had elaborate ML dendritic trees covered with dendritic spines. In 26-mo.-old mice the dendrites had many varicosities and fewer short blunted dendritic spines. Large multipolar neurons in older mice had thinner dendrites with more varicosities than were in the 3-mo.-old group. In both age groups, multipolar cells had few dendritic spines distally limited. Small and large spherical cells had 2-5 primary dendrites with varicosities, little higher-order branching and spines. Fusiform cells had 1 or 2 primary dendrites, little secondary branching and few to no spines. Minor degenerative changes were noted in spherical and fusiform cells in the 2 age groups. These included dendritic varicosities, interruptions and some irregularities of somata surface. Degenerative changes present in the cochlea had significant effects on a limited population of DCN neurons. The neuronal morphology and architecture of the DCN in C57BL/6 mouse is similar to other mammalian species.