Traditionally used Thai medicinal plants: In vitro anti-inflammatory, anticancer and antioxidant activities
Traditionally used Thai medicinal plants: In vitro anti-inflammatory, anticancer and antioxidant activities
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DOI：
10.1016/j.jep.2010.04.036
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发表时间：
2010-07-20
影响因子：
5.4
通讯作者：
Heinrich, Michael
中科院分区：
文献类型：
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作者：
Siriwatanametanon, Nisarat;Fiebich, Bernd L.;Heinrich, Michael
研究目的：为了评估传统的泰国关于药用植物的治疗潜力的说法，并为未来的植物化学研究选择植物，从泰国教科书中选择了9种具有抗炎用途的植物物种，并评估了它们的体外抗炎，抗增殖和抗氧化活性。核因子-κ b通过荧光素酶测定确定稳定转染的HeLa细胞中的（NF-κ B）抑制作用，并通过ELISA评估对原代单核细胞中LPS诱导的促炎介质前列腺素E2（PGE 2）、白细胞介素（IL）-6、IL-1 β和肿瘤坏死因子（INF）α的影响。采用MTT法和XTT法检测了其对HeLa细胞、人白血病CCRF-CEM细胞和多药耐药CEM/ADR 5000亚系的细胞毒活性。然而，氧化还原状态已与炎症和癌症，抗氧化作用也进行了评估，使用DPPH，脂质过氧化和Folin-Ciocalteau方法。硬毛木和木蝴蝶表现出最有希望的NF-κ B抑制作用，具有最低的IC 50值（分别为41.96和47.45 μ g/ml）。Muehlenbeckia platyclada不抑制NF-κ B的活化，但有效地抑制IL-6的释放。IL-1 β和TNF-α的IC 50值范围为0.28 - 8.67 μ g/ml。Pouzolzia indica对CCRF-CEM细胞和多药耐药CEM/ADR 5000细胞的细胞毒性最强（10 μ g/ml时的存活率分别为9.75%和10.48%）。鼻刺花对HeLa细胞的细胞毒性最强（IC 50 3.63 μ g/ml），对多药耐药CEM/ADR 5000细胞显示出特异性细胞毒性（10 μ g/ml时存活率为18.72%，与其对CCRF-CEM细胞的细胞毒性相比，p < 0.0001）。此外，木蝴蝶表现出高水平的抗氧化活性，通过抑制脂质过氧化（IC 50 0.08 μ g/ml）。结论：这项研究提供了在体外使用的泰国植物，最重要的是菊三七变种的证据。Hispida，Oroxylum indicum和Muehlenbeckia platyclada作为泰国抗炎药，这些植物现在是进一步植物化学研究的优先事项。(C)2010爱思唯尔爱尔兰有限公司版权所有。
Aims of the study: In order to assess traditional Thai claims about the therapeutic potential of medicinal plants and to select plants for future phytochemical research, nine plant species with anti-inflammatory uses were selected from Thai textbooks and assessed for their in vitro anti-inflammatory, antiproliferative and antioxidant activities.Methods: Nuclear factor-kappaB (NF-kappa B) inhibitory effects in stably transfected HeLa cells were determined by luciferase assay, and effects on LPS-induced pro-inflammatory mediators prostaglandin E2 (PGE2), interleukin (IL)-6, IL-1 beta, and tumour necrosis factor (INF)alpha in primary monocytes were assessed by ELISA. Cytotoxic activities were examined against HeLa cells, human leukaemia CCRF-CEM cells and the multidrug-resistant CEM/ADR5000 subline using the MTT and XTT tests. However, a redox status has been linked with both inflammation and cancer, antioxidant effects were also assessed using the DPPH, lipid-peroxidation, and Folin-Ciocalteau methods.Results: Among all the nine species, Gynura pseudochina var. hispida and Oroxylum indicum showed the most promising NF-kappa B inhibitory effects with the lowest IC50 values (41.96 and 47.45 mu g/ml, respectively). Muehlenbeckia platyclada did not inhibit the NF-kappa B activation but effectively inhibited the release of IL-6. IL-1 beta and TNF-alpha with IC50 values ranging between 0.28 and 8.67 mu g/ml. Pouzolzia indica was the most cytotoxic against CCRF-CEM cells and the multidrug-resistant CEM/ADR5000 cells (9.75% and 10.48% viability, at 10 mu g/ml, respectively). Rhinacanthus nasutus was the most potent cytotoxicity against HeLa cells (IC50 3.63 mu g/ml) and showed specific cytotoxicity against the multidrug-resistant CEM/ADR5000 cells (18.72% viability at 10 mu g/ml, p < 0.0001 when compared to its cytotoxicity against CCRF-CEM cells). Moreover, Oroxylum indicum showed a high level of antioxidant activity by inhibiting lipid-peroxidation (IC50 0.08 mu g/ml).Conclusions: This study provides in vitro evidence for the use of the Thai plants, most importantly Gynura pseudochina var. hispida, Oroxylum indicum and Muehlenbeckia platyclada as Thai anti-inflammatory remedies and these plants are now a priority for further phytochemical research. (C) 2010 Elsevier Ireland Ltd. All rights reserved.