Prostaglandin F2alpha regulates distinct physiological changes in early and mid-cycle bovine corpora lutea.
Prostaglandin F2alpha regulates distinct physiological changes in early and mid-cycle bovine corpora lutea.
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前列腺素 F2α 调节早期和中期牛黄体的明显生理变化。
DOI：
10.1095/biolreprod58.2.346
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发表时间：
1998
影响因子：
3.6
通讯作者：
Wiltbank,MC
中科院分区：
文献类型：
--
作者：
Tsai,SJ;Wiltbank,MC
前列腺素 (PG) F2α 是大多数物种中的主要木黄素溶血素。尽管存在相似浓度的高亲和力 PGF2α 受体（FP 受体），但 PGF2α 的单次治疗将导致周期中期而非周期早期（发情后第 1-5 天）牛黄体 (CL) 的消退。本研究旨在确定 PGF2α 是否在早期和中期 CL 中激活类似的细胞内过程。奶牛在 LH 激增（由 GnRH 注射诱导）发生后第 4 或 11 天接受盐水或 25 mg PGF2α 注射（肌肉注射；n = 6/组），并在治疗后 4 小时收集 CL。正如预期的那样，PGF2α 治疗后 4 小时，CL 体积和黄体重量没有差异。在用 PGF2α 处理的第 4 天和第 11 天的 CL 中，黄体维生素 C 浓度以及 3β-羟基类固醇脱氢酶和 FP 受体 mRNA 的稳态浓度均降低了 4 小时（p < 0.05）。这些结果表明 PGF2α 在早期 CL 中具有明显的作用。相比之下，在中期 CL 中，编码 PG G/H 合酶-2 (PGHS-2) 的 mRNA 的稳态浓度因使用 PGF2α 治疗而增加，但在早期周期 CL 中因 PGF2α 治疗而降低 (p < 0.05)。此外，用PGF2α治疗周期中期而非周期早期的奶牛，黄体和血清孕酮浓度降低了4小时（p<0.05）。总之，PGF2α 在牛 CL 的早期和中期均明显发挥作用。早期 CL 中缺乏 PGF2α 诱导的黄体分解可能是由于基因表达的特定变化，特别是 PGHS-2，这可能会阻止黄体内 PGF2α 的产生以及可能的其他关键黄体分解过程。
Prostaglandin (PG) F2αis the primary luteolysin in most species. A single treatment with PGF2αwill cause regression of the mid-cycle but not the early-cycle (Days 1-5 after estrus) bovine corpus luteum (CL) despite the presence of similar concentrations of high-affinity PGF2αreceptors (FP receptors). This study was designed to determine whether PGF2αactivated similar intracellular processes in early- and mid-cycle CL. Cows received saline or 25 mg PGF2αinjection (i.m.; n = 6/group) on Day 4 or 11 after onset of the LH surge (induced by GnRH injection), and CL were collected at 4 h after treatment. As expected, CL volumes and luteal weights were not different at 4 h after PGF2αtreatment. Luteal vitamin C concentration and steady-state concentrations of mRNA for 3β-hydroxysteroid dehydrogenase and for FP receptor were decreased by 4 h in both Day 4 and 11 CL treated with PGF2α(p< 0.05). These results demonstrate clear actions of PGF2αin the early CL. In contrast, steady-state concentrations of mRNA encoding PG G/H synthase-2 (PGHS-2) were increased by treatment with PGF2αin mid-cycle CL but decreased by PGF2αin early-cycle CL (p< 0.05). In addition, treatment of mid-cycle but not early-cycle cows with PGF2αdecreased luteal and serum progesterone concentrations by 4 h (p< 0.05). In summary, PGF2αclearly exerts actions in both early- and mid-cycle bovine CL. The lack of PGF2α-induced luteolysis in the early CL may be due to specific changes in gene expression, especially PGHS-2, that may prevent intraluteal PGF2αproduction and possibly other key luteolytic processes.