SEPSIS DOES NOT ALTER RED-BLOOD-CELL GLUCOSE-METABOLISM OR NA+ CONCENTRATION - A H-2-NMR,NA-23-NMR STUDY
SEPSIS DOES NOT ALTER RED-BLOOD-CELL GLUCOSE-METABOLISM OR NA+ CONCENTRATION - A H-2-NMR,NA-23-NMR STUDY
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DOI：
10.1152/ajpregu.1990.258.1.r21
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发表时间：
1990-01-01
影响因子：
--
通讯作者：
KARL, IE
中科院分区：
文献类型：
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作者：
HOTCHKISS, RS;SONG, SK;KARL, IE
通过使用 23Na-和 2H-核磁共振 (NMR) 光谱检查脓毒症对大鼠红细胞 (RBC) 细胞内 Na+ 浓度 ([Na+]i) 和葡萄糖代谢的影响。使用盲肠结扎和穿孔技术在 15 只氟烷麻醉的雌性 Sprague-Dawley 大鼠中诱导脓毒症； 14 只对照大鼠接受盲肠操作但未结扎。将动物禁食36小时，但允许自由饮水。术后36小时，使用三聚磷酸镝作为化学位移试剂，通过23Na-NMR检查红细胞。还检查了 17 名危重非脓毒症患者和 7 名诊断为脓毒症患者的人类红细胞中的 [Na+]i。通过 23Na-NMR 和电感耦合等离子体原子发射光谱 (ICP-AES) 测定了五只大鼠红细胞样本的 [Na+]i。对于葡萄糖代谢研究，将来自脓毒症大鼠和对照大鼠的红细胞悬浮在含有[6,6-2H2]葡萄糖的改良Krebs-Henseleit缓冲液中，并通过2H-NMR进行检查。在对照组或脓毒症大鼠的红细胞中，[Na+]i 或葡萄糖利用率没有发现显着差异。两组患者的[Na+]i无差异。通过 NMR 光谱测定的 [Na+]i 与使用 ICP-AES 的测量结果非常一致，并确定 NMR 可以看到红细胞中 100% 的 [Na+]i。通过 2H-NMR 测定的葡萄糖测量值与酶分析密切相关（校正系数 = 0.93）。这些研究表明没有证据表明脓毒症扰乱了红细胞膜功能或代谢。
The effects of sepsis on intracellular Na+ concentration ([Na+]i) and glucose metabolism were examined in rat red blood cells (RBCs) by using 23Na- and 2H-nuclear magnetic resonance (NMR) spectroscopy. Sepsis was induced in 15 halothane-anesthetized female Sprague-Dawley rats by using the cecal ligation and perforation technique; 14 controls rats underwent cecal manipulation without ligation. The animals were fasted for 36 h, but allowed free access to water. As 36 h postsurgery, RBCs were examined by 23Na-NMR by using dysprosium tripolyphosphate as a chemical shift reagent. Human RBCs from 17 critically ill nonseptic patients and from 7 patients who were diagnosed as septic were also examined for [Na+]i. Five rat RBC specimens had [Na+]i determined by both 23Na-NMR and inductively coupled plasma-atomic emission spectroscopy (ICP-AES). For glucose metabolism studies, RBCs from septic and control rats were suspended in modified Krebs-Henseleit buffer containing [6,6-2H2]glucose and examined by 2H-NMR. No significant differences in [Na+]i or glucose utilization were found in RBCs from control or septic rats. There were no differences in [Na+]i in the two groups of patients. The [Na+]i determined by NMR spectroscopy agreed closely with measurements using ICP-AES and establish that 100% of the [Na+]i of the RBC is visible by NMR. Glucose measurements determined by 2H-NMR correlated closely (correction coefficient = 0.93) with enzymatic analysis. These studies showed no evidence that sepsis disturbed RBC membrane function or metabolism.