METABOLISM OF D-SERINE IN ESCHERICHIA-COLI K-12 - MECHANISM OF GROWTH INHIBITION
METABOLISM OF D-SERINE IN ESCHERICHIA-COLI K-12 - MECHANISM OF GROWTH INHIBITION
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DOI：
10.1128/jb.114.2.685-694.1973
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发表时间：
1973-01-01
影响因子：
3.2
通讯作者：
MCFALL, E
中科院分区：
文献类型：
--
作者：
COSLOY, SD;MCFALL, E
在没有明显杀伤作用的情况下，浓度大于50 μg/ml的d-丝氨酸抑制不能形成d-丝氨酸脱氨酶的大肠杆菌K-12突变体在基本培养基中的生长。突变体最终在较低浓度下恢复。没有证据表明d-丝氨酸在丰富的培养基中具有毒性。d-丝氨酸不干扰l-丝氨酸的活化、一碳代谢或磷脂酰丝氨酸的形成。然而，Pizer（个人交流）发现它是丝氨酸生物合成第一种酶的强大反馈抑制剂。在l-丝氨酸存在的情况下，残留毒性在很大程度上被泛酸非竞争性地克服，表明d-丝氨酸通过影响两个靶点来抑制生长：泛酸生物合成和l-丝氨酸生物合成。coliK-12。污染l-丝氨酸和d-丝氨酸制剂有助于d-丝氨酸抑制反应。
Without significant killing,d-serine at concentrations greater than 50 μg/ml inhibits growth in minimal media of mutants ofEscherichia coliK-12 unable to formd-serine deaminase. The mutants eventually recover at lower concentrations. There is no evidence ofd-serine toxicity in rich media. Toxicity is partially reversed byl-serine.d-Serine does not interfere withl-serine activation, one-carbon metabolism, or (Cronan, personal communication) formation of phosphatidylserine. Pizer (personal communication) finds, however, that it is a powerful feedback inhibitor of the first enzyme ofl-serine biosynthesis. In the presence ofl-serine, the residual toxicity is largely and noncompetitively over come by pantothenate, indicating thatd-serine inhibits growth by affecting two targets: pantothenate biosynthesis andl-serine biosynthesis.l-Serine causes transient growth inhibition inE. coliK-12. Contaminatingl-serine ind-serine preparations contributes to thed-serine inhibitory response.