EXOSPORIUM AND SPORE COAT FORMATION IN BACILLUS-CEREUS-T
EXOSPORIUM AND SPORE COAT FORMATION IN BACILLUS-CEREUS-T
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DOI：
10.1128/jb.115.3.1179-1190.1973
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发表时间：
1973-01-01
影响因子：
3.2
通讯作者：
MURRELL, WG
中科院分区：
文献类型：
--
作者：
OHYE, DF;MURRELL, WG
蜡状芽孢杆菌（BacilluscereusT）的孢子外壁是一个位于孢子囊中部的细胞质中的小薄层，靠近孢子囊外前膜（OFSM）。连续切片、各种染色方法和酶处理均未能显示小板层与OFSM之间的任何联系。孢子外壁的前缘向细胞的极端移动，直到孢子被完全包裹。中涂层形成于孢子外壁和OFSM之间，由三层单板或“带”组成，由两个电子致密层和一个电子透明层隔开。这条“带”通常首先在孢子囊的中心被观察到，在前孢子表面上的发育没有改变厚度或外观。在中被毛和外胚层之间，一层约50 nm厚的细胞质被发育中的被毛包围，这层细胞质成为内被毛。将电子致密材料沉积在中间涂层的外表面上以形成外涂层。
The exosporium ofBacillus cereusT was first observed as a small lamella in the cytoplasm in proximity to the outer forespore membrane (OFSM) near the middle of the sporangium. Serial sections, various staining methods, and enzyme treatments failed to show any connections between the small lamella and the OFSM. The advancing edge of the exosporium moved toward the polar end of the cell until the spore was completely enveloped. The middle coat was formed between the exosporium and the OFSM from a three-layered single plate or “belt,” consisting of two electron-dense layers separated by an electron-transparent layer. This “belt,” usually first observed toward the center of the sporangium, developed without changing thickness or appearance over the surface of the forespore. Between the middle coat and the OFSM, a layer of cytoplasm about 50-nm thick was enclosed by the developing coat; this became the inner coat. Electron-dense material was deposited on the outer surface of the middle coat to form the outer coat.