Gene expression analysis of plant host-pathogen interactions by SuperSAGE
Gene expression analysis of plant host-pathogen interactions by SuperSAGE
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DOI：
10.1073/pnas.2536670100
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发表时间：
2003-12-23
影响因子：
11.1
通讯作者：
Terauchi, R
中科院分区：
文献类型：
--
作者：
Matsumura, H;Reich, S;Terauchi, R
III型限制性内切酶EcoP15I用于从cdna的指定位置分离26bp的片段。我们将这种对传统基因表达序列分析（SAGE）程序进行了实质性改进的变体称为“SuperSAGE”。将SuperSAGE应用于稻瘟病病水稻叶片，利用稻瘟病病寄主和稻瘟病真菌的全基因组序列，同时监测两种寄主和稻瘟病真菌的基因表达谱，发现疏水酶基因是稻瘟病病水稻叶片中转录最活跃的稻瘟病菌基因。此外，SuperSAGE被应用于研究所谓的过敏反应前的基因表达变化，在INF1诱导剂处理的烟叶中，这是一种没有DNA数据库的“非模型”生物。再一次，SuperSAGE允许快速识别由激发子上调或下调的基因。令人惊讶的是，许多下调的基因编码了参与光合作用的蛋白质。对于两种或多种相互作用的生物体，如宿主和病原体，以及没有DNA数据库的生物体的转录组分析，SuperSAGE将特别有用。
The type III restriction endonuclease EcoP15I was used in isolating fragments of 26 bp from defined positions of cDNAs. We call this substantially improved variant to the conventional serial analysis of gene expression (SAGE) procedure "SuperSAGE." By applying SuperSAGE to Magnaporthe grisea (blast)-infected rice leaves, gene expression profiles of both the rice host and blast fungus were simultaneously monitored by making use of the fully sequenced genomes of both organisms, revealing that the hydrophobin gene is the most actively transcribed M. grisea gene in blast-infected rice leaves. Moreover, SuperSAGE was applied to study gene expression changes before the so-called hypersensitive response in INF1 elicitor-treated Nicotiana benthamiana, a "nonmodel" organism for which no DNA database is available. Again, SuperSAGE allowed rapid identification of genes up- or down-regulated by the elicitor. Surprisingly, many of the down-regulated genes coded for proteins involved in photosynthesis. SuperSAGE will be especially useful for transcriptome profiling of two or more interacting organisms like hosts and pathogens, and of organisms, for which no DNA database is available.