DIRECT MEASUREMENT OF RADIATION SENSITIVITY OF NORMAL MOUSE BONE MARROW CELLS
DIRECT MEASUREMENT OF RADIATION SENSITIVITY OF NORMAL MOUSE BONE MARROW CELLS
复制标题
DOI：
10.2307/3570892
复制
发表时间：
1961-01-01
影响因子：
3.4
通讯作者：
MCCULLOCH, EA
中科院分区：
文献类型：
--
作者：
TILL, JE;MCCULLOCH, EA
已经开发了一种技术，用于估计小鼠骨髓悬浮液中能够持续增殖的细胞数量。将各种稀释的骨髓悬浮液静脉注射到2-4个月大的同种试验动物体内，用950倍x射线（全身）预照射，该剂量在实验期间阻止脾脏骨髓细胞的肉眼或显微镜下再生。10天后检查这些动物的脾脏时，可以看到增殖的造血细胞菌落。在大约十倍的细胞浓度范围内，菌落是离散的，可以宏观地枚举。由此确定的菌落数量与注射的细胞数量呈线性关系。注射105个未辐照的有核骨髓细胞后，平均每个脾脏形成约10个菌落。利用该技术，获得了正常小鼠骨髓细胞在体外辐照而在体内增殖的辐射存活曲线。这条曲线的平均致死剂量（MLD）为115[正负]8拉德（Co60伽马射线），命中数约为2。对C3Hf和C57BI小鼠的实验也得到了类似的结果。
A technique has been developed for estimating the number of cells in a suspension of mouse bone marrow capable of continued proliferation. Various dilutions of the marrow suspension are injected intravenously into 2-4-month-old isologous test animals, pre-irradiated (total body) with 950 of X-rays, a dose that prevents gross or microscopic regeneration of marrow cells in the spleen during the period of the experiment. When the spleens of these animals are examined after 10 days, colonies of proliferating hematopoietic cells are seen. Over approximately a tenfold range of cell concentration, colonies are discrete and may be enumerated macroscopic ally. The number of colonies thus determined is linearly related to the number of cells injected. On the average, about 10 colonies per spleen are formed when 105 unirradiated, nucleated marrow cells are injected. Using this technique, a radiation survival curve has been obtained for normal mouse bone marrow cells irradiated in vitro but proliferating in vivo. The curve has a mean lethal dose (MLD) of 115 [plus or minus] 8 rads ( of Co60 gamma rays) and a hit number of about 2. Experiments on C3Hf and C57BI mice yielded similar results.