Skeletal site-specific characterization of orofacial and iliac crest human bone marrow stromal cells in same individuals
Skeletal site-specific characterization of orofacial and iliac crest human bone marrow stromal cells in same individuals
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DOI：
10.1016/j.bone.2005.10.027
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发表时间：
2006-06-01
期刊：
影响因子：
4.1
通讯作者：
Robey, Pamela G.
中科院分区：
文献类型：
--
作者：
Akintoye, Sunday O.;Lam, Thanh;Robey, Pamela G.
轴向和阑尾骨骼的自体移植物通常用于修复类似骨缺损的骨骼通常会导致不利的结果。这种临床观察结果以及许多骨异常仅限于颅骨的事实表明，口面，轴向和阑尾骨骼的骨代谢存在显着差异。这些差异是由胚胎祖细胞的位点特异性和居民多能人体骨髓基质细胞（HBMSC）决定的。这项研究研究了体外（上颌骨和下颌骨）和轴向（iLiac Crest）HBMSC在体外和体内之间的骨骼位点特异性表型和功能差异。具有和不具有成骨诱导剂的基本较高，下颌骨和iLiac Crest HBMSC的主要培养物。位点特异性的表征包括菌落形成效率，细胞增殖，衰老前的寿命，表面标记的相对存在，脂肪生成，成骨和移植在免疫功能低下的小鼠中以比较骨骼再生能力。与iLiac Crest细胞相比，口面HBMSC（mscs）随着衰老的延迟增殖，表达了较高水平的碱性磷酸酶，并且在体外表现出更多的钙积累。从三个骨骼部位分离的细胞对于HBMSC的标记stro 1的阳性呈阳性。 MSC在体内形成更多的骨，而iLiac Crest HBMSC形成了更紧凑的骨骼，包括造血组织，并且在体外和体内对成骨和脂肪源性诱导的反应性更高。这些数据表明，来自同一个体的HBMSC在体外和体内以特定于骨骼的特定方式不同，并将口面骨髓基质细胞确定为独特的细胞群体。需要进一步了解HBMSC的位点特异性及其对现场特异性骨骼疾病和再生的影响。由Elsevier Inc.出版
Autologous grafts from axial and appendicular bones commonly used to repair orofacial bone defects often result in unfavorable outcome. This clinical observation, along with the fact that many bone abnormalities are limited to craniofacial bones, suggests that there are significant differences in bone metabolism in orofacial, axial and appendicular bones. It is plausible that these differences are dictated by site-specificity of embryological progenitor cells and osteogenic properties of resident multipotent human bone marrow stromal cells (hBMSCs). This study investigated skeletal site-specific phenotypic and functional differences between orofacial (maxilla and mandible) and axial (iliac crest) hBMSCs in vitro and in vivo. Primary cultures of maxilla, mandible and iliac crest hBMSCs were established with and without osteogenic inducers. Site-specific characterization included colony forming efficiency, cell proliferation, life span before senescence, relative presence of surface markers, adipogenesis, osteogenesis and transplantation in immunocompromised mice to compare bone regenerative capacity. Compared with iliac crest cells, orofacial hBMSCs (OF-MSCs) proliferated more rapidly with delayed senescence, expressed higher levels of alkaline phosphatase and demonstrated more calcium accumulation in vitro. Cells isolated from the three skeletal sites were variably positive for STRO 1, a marker of hBMSCs. OF-MSCs formed more bone in vivo, while iliac crest hBMSCs formed more compacted bone that included hematopoietic tissue and were more responsive in vitro and in vivo to osteogenic and adipogenic inductions. These data demonstrate that hBMSCs from the same individuals differ in vitro and in vivo in a skeletal site-specific fashion and identified orofacial marrow stromal cells as unique cell populations. Further understanding of site-specific properties of hBMSCs and their impact on site-specific bone diseases and regeneration are needed. Published by Elsevier Inc.