The Impact of Matrix Metalloproteinases and Their Tissue Inhibitors in Inflammatory Bowel Diseases
The Impact of Matrix Metalloproteinases and Their Tissue Inhibitors in Inflammatory Bowel Diseases
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DOI：
10.1159/000336995
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发表时间：
2012-01-01
影响因子：
2.3
通讯作者：
Herszenyi, Laszlo
中科院分区：
文献类型：
--
作者：
Lakatos, Gabor;Hritz, Istvan;Herszenyi, Laszlo
背景：基质金属蛋白酶(MMPs)可能在炎症性肠病(IBD)的发病机制中发挥作用。然而，在相同的实验环境下，很少有人研究血清MMPs及其抑制物[金属蛋白酶组织抑制物(TIMPs)]对溃疡性结肠炎(UC)和克罗恩病(CD)的影响以及它们与IBD活动的相关性。方法：采用双抗体夹心酶联免疫分析法检测23例UC、25例CD患者和10例健康对照血清中MMP2、MMP7、MMP9、TIMP-1和TIMP-2的水平。统计分析采用单因素方差分析和学生t检验。使用线性回归分析或Spearman‘s r检验来评估相关性。P<；0.05的差异被认为是显著的。结果：UC和CD患者血清中MMP9、TIMP-1和TIMP-2的抗原浓度均显著高于对照组。CD患者血清中MMP7的表达也明显高于正常对照组。MMP9、TIMP-1抗原水平升高与IBD病情活动呈显著正相关。MMP-2和TIMP-2水平与CD活性呈负相关。UC和CD组织中MMP9/TIMP-1和MMP2/TIMP-2抗原水平均呈显著正相关。结论：UC和CD患者血清中MMP9、TIMP-1和TIMP-2抗原水平较正常对照组显著升高。我们的结果提示MMPs和TIMP可能参与了IBD的炎症和重塑过程。血清MMP9和TIMP 1可作为评估IBD活动性的附加生物标志物。版权所有(C)2012 S.Karger AG，巴塞尔
Background: It has been suggested that matrix metalloproteinases (MMPs) may play a role in the pathogenesis of inflammatory bowel diseases (IBD). However, the impact of serum MMPs and their inhibitors [tissue inhibitors of metalloproteinases (TIMPs)] have scarcely been investigated in the same experimental setting in ulcerative colitis (UC) and Crohn's disease (CD) as well as their correlation with IBD activity. Methods: MMP-2, MMP-7, MMP-9, TIMP-1 and TIMP-2 serum antigen levels were determined in 23 patients with UC, 25 patients with CD and 10 healthy control patients by enzyme-linked immunoassay technique. Statistical analysis with one-way ANOVA and Student's t tests was performed. A linear regression analysis or a Spearman's r test was used to assess correlation. Differences were considered significant with p < 0.05. Results: Serum antigen concentrations of MMP-9, TIMP-1 and TIMP-2 were significantly higher in UC and CD patients compared to controls. MMP-7 was also significantly higher in CD compared with controls. Elevated MMP-9 and TIMP-1 antigen levels showed significant positive correlation with disease activity of IBD. MMP-2 and TIMP-2 levels inversely correlated with CD activity. Significant correlations were found between MMP-9/TIMP-1 and MMP-2/TIMP-2 antigen levels in both UC and CD. Conclusions: We demonstrated that serum antigen concentrations of MMP-9, TIMP-1 and TIMP-2 were significantly increased in patients with UC and CD compared to controls. Our results suggest that MMPs and TIMPs may contribute to the inflammatory and remodeling processes in IBD. Serum MMP-9 and TIMP-1 might be useful as additional biomarkers in the assessment of IBD activity. Copyright (C) 2012 S. Karger AG, Basel