The quantitation of a native chondroitin sulfate epitope in synovial fluid lavages and articular cartilage from canine experimental osteoarthritis and disuse atrophy.
The quantitation of a native chondroitin sulfate epitope in synovial fluid lavages and articular cartilage from canine experimental osteoarthritis and disuse atrophy.
复制标题
犬实验性骨关节炎和废用性萎缩的滑液灌洗液和关节软骨中天然硫酸软骨素表位的定量。
DOI：
10.1002/art.1780360416
复制
发表时间：
1993
影响因子：
--
通讯作者：
Caterson,B
中科院分区：
文献类型：
--
作者：
Ratcliffe,A;Shurety,W;Caterson,B
目的：先前的研究表明，在实验性早期骨关节炎(OA)犬膝关节的关节软骨蛋白多糖中存在天然的硫酸软骨素表位，但在正常软骨中不存在。本研究的目的是定量检测单抗3-B-3在病变关节滑液和关节软骨中识别的天然表位。方法：建立了识别天然软骨素-6-硫酸盐结构的单抗3-B-3的免疫分析方法，并用于分析早期实验性骨关节炎或轻度废用性萎缩的犬膝关节和对照动物的关节液灌洗材料和关节软骨提取物。结论关节软骨和滑液中天然的3-B-3表位可能是早期退行性关节疾病中正在发生的合成代谢事件的特异性标志物。
Objective.Previous studies have shown the presence of a native chondroitin sulfate epitope in articular cartilage proteoglycans from canine knee joints with experimental early osteoarthritis (OA), but not in normal cartilage. The objective of this study was to quantitate the native epitope recognized by monoclonal antibody 3‐B‐3 in synovial fluids and articular cartilage of diseased joints.Methods.An immunoassay with monoclonal antibody 3‐B‐3, which recognizes a native chondroitin‐6‐sulfate structure, was developed and used to analyze synovial fluid lavage material and extracts of articular cartilage from canine knee joints with early experimental OA or with mild disuse atrophy, and from control animals.Results.The concentration of epitope in the OA fluids was elevated 33–35‐fold, and in the OA articular cartilage extracts it was elevated > 200‐fold, compared with samples from the control group. No significant difference was detected in the levels of 3‐B‐3 epitope in the synovial fluid lavage material or cartilage extracts from the joints of the disuse group versus the control group.Conclusion.The native 3‐B‐3 epitope in articular cartilage and synovial fluids may be a specific marker of ongoing anabolic events in early degenerative joint disease.