Targeting, insertion, and localization of Escherichia coli YidC
Targeting, insertion, and localization of Escherichia coli YidC
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DOI：
10.1074/jbc.m200311200
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发表时间：
2002-04-12
影响因子：
4.8
通讯作者：
Luirink, J
中科院分区：
文献类型：
--
作者：
Urbanus, ML;Fröderberg, L;Luirink, J
YidC最近被证明在内膜蛋白(IMP)的组装中发挥重要作用，这些IMP既与SEC转位蛋白结合又与SEC转位蛋白分离。YidC本身是一种多面性IMP，对其生物发生、结构和功能特性知之甚少。在这里，我们用体内和体外的方法分析了YIDC的靶向性和膜整合。联合数据表明，YidC是信号识别颗粒的靶点，并在生物发生早期插入到SecAYEG-YidC易位子，不同于其线粒体同源物Oxa1p。此外，与参与IMP组装的其他成分相比，YidC的含量相对较高，并且主要定位于细胞的两极。
YidC was recently shown to play an important role in the assembly of inner membrane proteins (IMPs) both in conjunction with and separate from the Sec-translocon. Little is known about the biogenesis and structural and functional properties of YidC, itself a polytopic IMP. Here we analyze the targeting and membrane integration of YidC using in vivo and in vitro approaches. The combined data indicate that YidC is targeted by the signal recognition particle and inserts at the SecAYEG-YidC translocon early during biogenesis, unlike its mitochondrial homologue Oxa1p. In addition, YidC is shown to be relatively abundant compared with other components involved in IMP assembly and is predominantly localized at the poles of the cell.