Lifeact: a versatile marker to visualize F-actin
Lifeact: a versatile marker to visualize F-actin
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DOI：
10.1038/nmeth.1220
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发表时间：
2008-07-01
期刊：
影响因子：
48
通讯作者：
Wedlich-Soldner, Roland
中科院分区：
文献类型：
--
作者：
Riedl, Julia;Crevenna, Alvaro H.;Wedlich-Soldner, Roland
肌动蛋白细胞骨架的实时成像对于研究许多基本的生物过程是至关重要的，但目前可视化肌动蛋白的方法有几个局限性。在这里，我们描述了Lifeact，一种17个氨基酸的多肽，它染色了真核细胞和组织中的丝状肌动蛋白(F-actin)结构。Lifeact在体外和体内不干扰肌动蛋白的动力学，其化学修饰的多肽形式允许在非转基因细胞中观察肌动蛋白的动力学。
Live imaging of the actin cytoskeleton is crucial for the study of many fundamental biological processes, but current approaches to visualize actin have several limitations. Here we describe Lifeact, a 17-amino-acid peptide, which stained filamentous actin (F-actin) structures in eukaryotic cells and tissues. Lifeact did not interfere with actin dynamics in vitro and in vivo and in its chemically modified peptide form allowed visualization of actin dynamics in nontransfectable cells.