Optical imaging in vitro provides evidence for the minicolumnar nature of cortical response
Optical imaging in vitro provides evidence for the minicolumnar nature of cortical response
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DOI：
10.1097/00001756-199711100-00019
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发表时间：
1997-11-10
期刊：
影响因子：
1.7
通讯作者：
Whitsel, BL
中科院分区：
文献类型：
--
作者：
Kohn, A;Pinheiro, A;Whitsel, BL
用内源性信号光学成像技术记录大鼠大脑皮层脑片VI层/白色物质边界对电刺激的反应。切片的光学响应是柱状的，从第VI层延伸到软膜表面，并且与同时记录的诱发电位的幅度强烈相关。光谱分析揭示了径向取向的空间变化的光信号的强度与周期为30-60 μ m/周期。尼氏染色切片也表现出径向取向的光密度周期性与相同的周期。我们得出结论，在固有的光信号的周期性变化对应于刺激激活的微柱。
THE response of rat neocortical slices to electrical stimulation at the layer VI/white matter border was recorded using intrinsic signal optical imaging. The optical response of the slice is column-shaped, extends from layer VI to the pial surface, and is strongly correlated with the amplitude of simultaneously recorded evoked potentials. Spectral analysis revealed radially oriented spatial variations in the intensity of the optical signal with a period of 30-60 mu m/cycle. Nissl-stained sections of slices also exhibited a radially oriented periodicity in optical density with the same period. We conclude that the periodic variations in the intrinsic optical signal correspond to stimulus-activated minicolumns.