Speckle rheological spectroscopy reveals wideband viscoelastic spectra of biological tissues.
Speckle rheological spectroscopy reveals wideband viscoelastic spectra of biological tissues.
复制标题
散斑流变光谱揭示了生物组织的宽带粘弹性光谱。
DOI：
10.1101/2023.06.08.544037
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Nadkarni,SeemantiniK
中科院分区：
文献类型：
--
作者：
Leartprapun,Nichaluk;Zeng,Ziqian;Hajjarian,Zeinab;Bossuyt,Veerle;Nadkarni,SeemantiniK
组织的机械转化不仅仅是一种症状，而是病理过程中的决定性驱动因素。组织由复杂的细胞网络、纤维蛋白和间质液组成，表现出明显的固体（弹性）和液体样（粘性）行为，其频率范围很广。然而，整个组织中宽带粘弹性行为的表征尚未被研究，在与基本细胞内过程和微观结构动力学相关的更高频率范围内留下了巨大的知识空白。在这里，我们提出宽带散斑流变光谱（剪切）来解决这一需求。我们首次在仿生支架和血凝块、乳腺肿瘤和骨骼的组织标本中，对频率相关的弹性模量和粘性模量进行了分析。通过在宽频谱范围内捕获以前无法获得的粘弹性行为，我们的方法提供了组织的独特而全面的机械特征，这可能提供新的机械生物学见解并为新的疾病预测提供信息。
Mechanical transformation of tissue is not merely a symptom but a decisive driver in pathological processes. Comprising intricate network of cells, fibrillar proteins, and interstitial fluid, tissues exhibit distinct solid-(elastic) and liquid-like (viscous) behaviours that span a wide band of frequencies. Yet, characterization of wideband viscoelastic behaviour in whole tissue has not been investigated, leaving a vast knowledge gap in the higher frequency range that is linked to fundamental intracellular processes and microstructural dynamics. Here, we present wideband Speckle rHEologicAl spectRoScopy (SHEARS) to address this need. We demonstrate, for the first time, analysis of frequency-dependent elastic and viscous moduli up to the sub-MHz regime in biomimetic scaffolds and tissue specimens of blood clots, breast tumours, and bone. By capturing previously inaccessible viscoelastic behaviour across the wide frequency spectrum, our approach provides distinct and comprehensive mechanical signatures of tissues that may provide new mechanobiological insights and inform novel disease prognostication.