Effect of follicle-stimulating hormone on insulin-like growth factor-I-stimulated rat granulosa cell deoxyribonucleic acid synthesis.
Effect of follicle-stimulating hormone on insulin-like growth factor-I-stimulated rat granulosa cell deoxyribonucleic acid synthesis.
复制标题
卵泡刺激素对胰岛素样生长因子-I刺激的大鼠颗粒细胞脱氧核糖核酸合成的影响。
DOI：
10.1210/endo.131.3.1380436
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发表时间：
1992
期刊：
影响因子：
4.8
通讯作者：
J. Barañao
中科院分区：
文献类型：
--
作者：
M. A. Bley;J. Simon;A. Estevez;L. de Asúa;J. Barañao
将来自己烯雌酚处理的未成熟大鼠的颗粒细胞在胶原包被的平板上的确定培养基中培养。胰岛素（ED50，656 +/- 110 ng/ml）和胰岛素样生长因子（IGF-I；ED50，95 +/- 10 ng/ml）显着增加胸苷掺入。磷酸二酯酶活性抑制剂甲基异丁基黄嘌呤可增强胰岛素和 IGF-I 的刺激作用。低剂量的 (Bu)2cAMP (0.2-1 mM) 也增强了两种肽的作用。相反，较高浓度具有抑制作用。同样，FSH 对胰岛素和 IGF-I 刺激的 DNA 合成产生双相增强。使用较低剂量 (2-20 ng/ml) 的促性腺激素可观察到最大效果（增加 2 至 6 倍）。 FSH 对 IGF-I 刺激的 DNA 合成的增强取决于细胞密度。最大相互作用需要 3-5 x 10(5) 个细胞/cm2 的铺板密度。结论是，FSH 通过 cAMP 介导的途径起作用，可以通过调节胰岛素和/或 IGF-I 的促有丝分裂作用来调节颗粒细胞增殖。
Granulosa cells from diethylstilbestrol-treated immature rats were cultured in a defined medium on collagen-coated plates. Thymidine incorporation was significantly increased by insulin (ED50, 656 +/- 110 ng/ml) and insulin-like growth factor (IGF-I; ED50, 95 +/- 10 ng/ml). Insulin and IGF-I stimulations were amplified by methylisobutylxanthine an inhibitor of phosphodiesterase activity. The effect of both peptides were also enhanced by low doses of (Bu)2cAMP (0.2-1 mM). In contrast, higher concentrations were inhibitory. Similarly, FSH produced a biphasic enhancement of the insulin- and IGF-I-stimulated DNA synthesis. Maximal effects (2- to 6-fold increases) were observed with the lower doses (2-20 ng/ml) of the gonadotropin. FSH enhancement of IGF-I-stimulated DNA synthesis was dependent on cell density. Plating densities of 3-5 x 10(5) cells/cm2 were required for a maximal interaction. It is concluded that FSH, acting through a cAMP-mediated pathway, may regulate granulosa cell proliferation by modulating the mitogenic effects of insulin and/or IGF-I.