Prenatal Radiation-Induced Limb Defects Mediated by Trp53-Dependent Apoptosis in Mice
Prenatal Radiation-Induced Limb Defects Mediated by Trp53-Dependent Apoptosis in Mice
复制标题
Trp53 依赖性细胞凋亡介导的产前辐射诱发的小鼠肢体缺陷
作者：
Bing Wang;H. Ohyama;K. Haginoya;T. Odaka;Takeshi Yamada;I. Hayata
摘要王，B.，Ohyama，H.，Haginoya，K.，Odaka，T.，Yamada，T.和Hayata，I. Trp 53依赖性细胞凋亡介导的小鼠产前放射诱导的肢体缺陷我们以前报道，在子宫内辐射诱导的胚胎肢芽趾前区的细胞凋亡是负责小鼠的数字缺陷。为了研究Trp 53基因的可能参与，本研究使用具有不同Trp 53状态的胚胎C57 BL/6 J小鼠进行。在趾前区和趾缺损中对辐射诱导的细胞凋亡的敏感性取决于Trp 53状态和辐射剂量;即，Trp 53野生型（Trp 53 +/+）小鼠似乎是最敏感的，Trp 53杂合型（Trp 53 +/-）小鼠是中等的，而Trp 53敲除型（Trp 53-/-）小鼠是最耐药的。这些结果表明，诱导细胞凋亡和指缺陷的产前照射在后期的器官发生是由Trp 53基因介导的。这些研究结果表明，野生型Trp 53基因可能是一个内在的遗传易感因素，是负责某些先天性缺陷引起的产前辐射。
Abstract Wang, B., Ohyama, H., Haginoya, K., Odaka, T., Yamada, T. and Hayata, I. Prenatal Radiation-Induced Limb Defects Mediated by Trp53-Dependent Apoptosis in Mice. We reported previously that in utero radiation-induced apoptosis in the predigital regions of embryonic limb buds was responsible for digital defects in mice. To investigate the possible involvement of the Trp53 gene, the present study was conducted using embryonic C57BL/6J mice with different Trp53 status. Susceptibility to radiation-induced apoptosis in the predigital regions and digital defects depended on both Trp53 status and the radiation dose; i.e., Trp53 wild-type (Trp53+/+) mice appeared to be the most sensitive, Trp53 heterozygous (Trp53+/–) mice were intermediate, and Trp53 knockout (Trp53–/–) mice were the most resistant. These results indicate that induction of apoptosis and digital defects by prenatal irradiation in the later period of organogenesis are mediated by the Trp53 gene. These findings suggest that the wild-type Trp53 gene may be an intrinsic genetic susceptibility factor that is responsible for certain congenital defects induced by prenatal irradiation.
影响因子：
11.2
作者：
Christoph H. Westphal;Katherine P Hoyes;C. Canman;Xin Huang;Michael B. Kastan;J. H. Hendry;Philip Leder
通讯作者：
Christoph H. Westphal;Katherine P Hoyes;C. Canman;Xin Huang;Michael B. Kastan;J. H. Hendry;Philip Leder
致畸剂和颅面畸形：与细胞死亡的关系。
DOI：
10.1242/dev.103.supplement.213
发表时间：
1988
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Sulik,KK;Cook,CS;Webster,WS
通讯作者：
Webster,WS
细胞凋亡与癌症治疗的关系。
DOI：
--
发表时间：
1994
期刊：
In vivo (Athens, Greece)
影响因子：
--
作者：
Milas,L;Stephens,LC;Meyn,RE
通讯作者：
Meyn,RE