A new dominant locus, E11, controls early flowering time and maturity in soybean
A new dominant locus, E11, controls early flowering time and maturity in soybean
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一个新的显性基因座 E11 控制大豆的早期开花时间和成熟度
DOI：
10.1007/s11032-019-0978-3
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发表时间：
2019-05-01
影响因子：
3.1
通讯作者：
Lu, Sijia
中科院分区：
文献类型：
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作者：
Wang, Feifei;Nan, Haiyang;Lu, Sijia
开花时间是一个由多基因控制的复杂生态性状，与光周期反应、成熟度和最终产量有关。为了进一步阐明开花时间的遗传机制，利用特异位点扩增片段测序（SLAF-Seq）技术，对影响开花时间和成熟度的数量性状基因座（QTL）进行了分析。总的来说，我们确定了3个QTL的染色体5，6，和7的重组自交系（RIL）的171个人之间的杂交Minsoy和Archer大豆的人口。在这些QTL中，一个位于第7染色体上的新QTL E11在RIL群体的F-6和F-8代的1.03Mb区域内同时被检测到，它解释了2a来总表型变异的15%。基因符号E11 e11已通过大豆遗传委员会审定。在E11位点剩余杂合系（RHL）中观察到的分离模式表明，早花由单个显性基因控制。该基因被精细定位到138 kb的区间，包括11个基于参考基因组的基因。通过氨基酸序列分析，确定了三个最可能的候选基因Glyma.07g048500、Glyma.07g049000和Glyma.07g049200。对两个近等基因系（NILs）的表型分析表明，在长日照（LD）条件下，E11等位基因的近等基因系比e11等位基因的近等基因系显著提高了开花时间和成熟度。这些结果表明，E11是一个新的开花时间基因，这将有助于提高我们对开花时间的机制和分子育种的认识。
Flowering time, as an important ecological trait related to photoperiod response, maturity, and final yield, is a complex trait conferred by multiple genes. To further elucidate the genetic mechanism for the flowering time, quantitative trait loci (QTLs) related to the flowering time and maturity were identified utilizing specific-locus amplified fragment sequencing (SLAF-Seq) technology. In total, we identified three QTLs on chromosomes 5, 6, and 7 from a recombinant inbred line (RIL) population of 171 individuals derived from a cross between Minsoy and Archer soybeans. Of these QTLs, one new QTL on chromosome 7, called E11, was simultaneously detected in an 1.03Mb region from the F-6 and F-8 generations of the RIL population, and accounted for 15% of the total phenotypic variation over 2years. The gene symbol E11e11 had been approved by the soybean genetic committee. The segregation patterns observed in residual heterozygous lines (RHLs) at the E11 locus revealed that early flowering was controlled by a single dominant gene. The gene was fine-mapped to an 138kb interval, including 11 genes based on the reference genome. Through amino acid sequence analysis, three most likely candidate genes, Glyma.07g048500, Glyma.07g049000, and Glyma.07g049200, were identified. The phenotypes detected from two near-isogenic lines (NILs) revealed that NILs for E11 allele significantly promoted the flowering time and maturity than NILs for the e11 under the long-day (LD) conditions. These results suggest that E11 is a new flowering time gene that will be valuable in improving our understanding of the mechanism for the flowering time and molecular breeding.