Crataegus pinnatifida polysaccharide alleviates colitis via modulation of gut microbiota and SCFAs metabolism
Crataegus pinnatifida polysaccharide alleviates colitis via modulation of gut microbiota and SCFAs metabolism
复制标题
山楂多糖通过调节肠道微生物群和短链脂肪酸 SCFA 代谢减轻结肠炎
DOI：
10.1016/j.ijbiomac.2021.03.137
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发表时间：
2021-03-31
影响因子：
8.2
通讯作者：
Ding, Kan
中科院分区：
文献类型：
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作者：
Guo, Ciliang;Wang, Yeqing;Ding, Kan
炎症性肠病（IBD）是一种常见的疾病，大多数药物都有副作用。山楂（山楂）是一种安全的药食同源植物，有报道对小鼠结肠炎有预防作用。然而，其生物活性成分和潜在的分子机制仍不清楚。在这里，我们建立了葡聚糖硫酸钠（DSS）诱导的小鼠结肠炎和从山楂中分离的多糖HAW 1 -2之间的直接联系。结果表明HAW 1 -2能修复结肠病变，抑制炎症细胞因子IL-1 β、IL-6和TNF-α的表达。同时，IKK α/β、IKB α、NF-κ B及磷酸化水平均受到明显抑制。提示HAW 1 -2具有减轻结肠炎的作用。此外，我们发现肠道微生物群的组成被改变，并且包括Alistipes和Odoribacter在内的拟杆菌属显著富集。此外，我们发现Alistipes和Odoribacter与乙酸和丙酸呈正相关，而与炎性细胞因子呈负相关。最后，我们证明HAW 1 -2的抗炎活性可能是由乙酸诱导的。总之，目前的数据显示HAW 1 -2可以直接改变肠道微生物群，特别是拟杆菌，并产生SCFA来抑制结肠炎。这也意味着IBD患者的微生物群定向干预应受到更多关注。(c)2021年由Elsevier B. V.出版
Inflammatory bowel disease (IBD) afflicted individual and most medications have side-effects. Crataegus pinnatifida (Hawthorn), which is a safe medicine and food homolog plant, has been reported to prevent colitis in murine. Yet the bioactivity component and the underlying molecular mechanism remain unclear. Here, we established a direct link between colitis induced by dextran sulphate sodium (DSS) in mice and polysaccharide HAW1-2 isolated from hawthorn. Our results showed HAW1-2 restored the pathological lesions in colon and inhibited the expression of inflammatory cytokines including IL-1 beta, IL-6 and TNF-alpha. Meanwhile, IKK alpha/beta, IKB alpha, NF-KB and the phosphorylation levels were inhibited significantly. These findings suggested HAW1-2 could alleviate the inflammation of colon. Further, we found the composition of gut microbiota was modified and Bacteroides including Alistipes and Odoribacter were significantly enriched. Besides, we showed Alistipes and Odoribacter were positively co-related with acetic acid and propionic acid while were negatively co-related with inflammatory cytokines. Finally, we demonstrated the anti-inflammation activity of HAW1-2 might be induced by acetic acid. Together, the present data revealed HAW1-2 could directly modify the gut microbiota, especially for Bacteroides, and generate SCFAs to inhibit colitis. It also implies microbiota-directed intervention in IBD patients should be particularly given more attention.(c) 2021 Published by Elsevier B.V.