The planar cell polarity protein Strabismus promotes Pins anterior localization during asymmetric division of sensory organ precursor cells in Drosophila
The planar cell polarity protein Strabismus promotes Pins anterior localization during asymmetric division of sensory organ precursor cells in Drosophila
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DOI：
10.1242/dev.00928
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发表时间：
2004-01-01
期刊：
影响因子：
4.6
通讯作者：
Schweisguth, F
中科院分区：
文献类型：
--
作者：
Bellaïche, Y;Beaudoin-Massiani, O;Schweisguth, F
细胞命运多样性的产生部分是由于在不对称的细胞分裂过程中细胞命运决定因素的不平等分离。在果蝇的刚毛谱系中，感觉器官前体(PI)细胞通过Frizzled型(FZ)受体信号沿前后轴(AP)极化。我们发现FZ在有丝分裂前定位于PI细胞的后尖皮质，而斜视(Stbm)和棘突(PK)也是PI细胞AP极化所必需的，共同定位于PI细胞的前尖皮质。因此，FZ、Stbm和PK的不对称定位在PI细胞有丝分裂之前定义了两个相反的皮质结构域。在有丝分裂时，Stbm形成一个前新月，与Inscuteable(Pins)和Discs-Large(DLG)的伙伴分布重叠，这两个成分是DLG-Pins-GOD复合体的两个组成部分，调节细胞命运决定因素的定位。在早期，STBM促进了Pins的前置定位。相比之下，杂乱无章的(Dsh)通过将Pins排除在后皮质而对抗Stbm。我们认为，依赖于STBM的Pins在PI细胞前皮质的募集是一种新的平面细胞极性读出。
Cell fate diversity is generated in part by the unequal segregation of cell-fate determinants during asymmetric cell division. In the Drosophila bristle lineage, the sensory organ precursor (pI) cell is polarized along the anteroposterior (AP) axis by Frizzled (Fz) receptor signaling. We show here that Fz localizes at the posterior apical cortex of the pI cell prior to mitosis, whereas Strabismus (Stbm) and Prickle (Pk), which are also required for AP polarization of the pI cell, co-localize at the anterior apical cortex. Thus, asymmetric localization of Fz, Stbm and Pk define two opposite cortical domains prior to mitosis of the pI cell. At mitosis, Stbm forms an anterior crescent that overlaps with the distribution of Partner of Inscuteable (Pins) and Discs-large (Dlg), two components of the anterior Dlg-Pins-God complex that regulates the localization of cell-fate determinants. At prophase, Stbm promotes the anterior localization of Pins. By contrast, Dishevelled (Dsh) acts antagonistically to Stbm by excluding Pins from the posterior cortex. We propose that the Stbm-dependent recruitment of Pins at the anterior cortex of the pI cell is a novel read-out of planar cell polarity.