Pyruvate kinase M2 is a PHD3-stimulated coactivator for hypoxia-inducible factor 1.
Pyruvate kinase M2 is a PHD3-stimulated coactivator for hypoxia-inducible factor 1.
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DOI：
10.1016/j.cell.2011.03.054
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发表时间：
2011-05-27
期刊：
影响因子：
64.5
通讯作者：
Semenza GL
中科院分区：
文献类型：
--
作者：
Luo W;Hu H;Chang R;Zhong J;Knabel M;O'Meally R;Cole RN;Pandey A;Semenza GL
The pyruvate kinase isoforms PKM1 and PKM2 are alternatively spliced products of the PKM2 gene. PKM2, but not PKM1, alters glucose metabolism in cancer cells and contributes to tumorigenesis by mechanisms that are not explained by its known biochemical activity. We show that PKM2 gene transcription is activated by hypoxia-inducible factor 1 (HIF-1). PKM2 interacts directly with the HIF-1α subunit and promotes transactivation of HIF-1 target genes by enhancing HIF-1 binding and p300 recruitment to hypoxia response elements, whereas PKM1 fails to regulate HIF-1 activity. Interaction of PKM2 with prolyl hydroxylase 3 (PHD3) enhances PKM2 binding to HIF-1α and PKM2 coactivator function. Mass spectrometry and anti-hydroxyproline antibody assays demonstrate PKM2 hydroxylation on proline-403/408. PHD3 knockdown inhibits PKM2 coactivator function, reduces glucose uptake and lactate production, and increases O2 consumption in cancer cells. Thus, PKM2 participates in a positive feedback loop that promotes HIF-1 transactivation and reprograms glucose metabolism in cancer cells.
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影响因子：
7.3
作者：
Xie L;Xiao K;Whalen EJ;Forrester MT;Freeman RS;Fong G;Gygi SP;Lefkowitz RJ;Stamler JS
通讯作者：
Stamler JS
影响因子：
8
作者：
Semenza, G. L.
通讯作者：
Semenza, G. L.
DOI：
10.1073/pnas.93.23.12969
发表时间：
1996-11-12
影响因子：
11.1
作者：
Arany, Z;Huang, LE;Livingston, DM
通讯作者：
Livingston, DM
影响因子：
5.3
作者：
Seagroves, TN;Ryan, HE;Johnson, RS
通讯作者：
Johnson, RS
影响因子：
4.8
作者：
Jiang, BH;Zheng, JZ;Semenza, GL
通讯作者：
Semenza, GL