The small GTP-binding protein Rho1p is localized on the Golgi apparatus and post-Golgi vesicles in Saccharomyces cerevisiae.
The small GTP-binding protein Rho1p is localized on the Golgi apparatus and post-Golgi vesicles in Saccharomyces cerevisiae.
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DOI：
10.1083/jcb.115.2.309
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发表时间：
1991-10
影响因子：
7.8
通讯作者：
Boquet, P
中科院分区：
文献类型：
--
作者：
McCaffrey, M;Johnson, J S;Goud, B;Myers, A M;Rossier, J;Popoff, M R;Madaule, P;Boquet, P
在酿酒酵母中，ras相关蛋白Rho 1 p基本上是肉毒梭菌胞外酶C3进行ADP核糖基化的唯一靶标。使用C3检测亚细胞组分中的Rho 1 p，发现Rho 1 p主要存在于含有大细胞器的10，000 g颗粒（P2）中;在100，000 g颗粒（P3）和细胞溶质中也检测到少量Rho 1 p。当P2细胞器分离在蔗糖密度梯度Rho 1 p共迁移与Kex-2活性，后期高尔基体标记。Rho 1 p的分布从P2转移到P3在几个突变体，积累后高尔基体囊泡。Rho 1 p comigrated与后高尔基体运输囊泡在分馏P3细胞器从野生型或sec 6细胞。含有Rho 1 p的囊泡大小相同，但密度不同，比那些轴承Sec 4p，ras相关蛋白位于后高尔基体囊泡和质膜。免疫荧光显微镜检测到Rho 1 p呈点状图案，信号集中在细胞周边和芽中。因此，在S.酿酒酵母Rho 1 p主要存在于高尔基体中，也存在于可能是早期高尔基体后囊泡的囊泡中。
In Saccharomyces cerevisiae the ras-related protein Rho1p is essentially the only target for ADP-ribosylation by exoenzyme C3 of Clostridium botulinum. Using C3 to detect Rho1p in subcellular fractions, Rho1p was found primarily in the 10,000 g pellet (P2) containing large organelles; small amounts also were detected in the 100,000 g pellet (P3), and cytosol. When P2 organelles were separated in sucrose density gradients Rho1p comigrated with the Kex-2 activity, a late Golgi marker. Rho1p distribution was shifted from P2 to P3 in several mutants that accumulate post-Golgi vesicles. Rho1p comigrated with post-Golgi transport vesicles during fractionation of P3 organelles from wild-type or sec6 cells. Vesicles containing Rho1p were of the same size but different density than those bearing Sec4p, a ras- related protein located both on post-Golgi vesicles and the plasma membrane. Immunofluorescence microscopy detected Rho1p as a punctate pattern, with signal concentrated towards the cell periphery and in the bud. Thus, in S. cerevisiae Rho1p resides primarily in the Golgi apparatus, and also in vesicles that are likely to be early post-Golgi vesicles.